Anti-PTPRC antibody (1260aa C-Term) (STJ140026)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 1, 260 aa to the C-terminus of human CD45 produced in E. coli.
General Information
| Short Description | Goat polyclonal Protein tyrosine phosphatase, receptor type, C (1260aa C-Term) antibody for use in WB, IHC-P and IHC-F in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and |
| Applications | WB/IHC-P/IHC-F |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IHC-F 1:250-1:1000IHC-P 1:250-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | PTPRC |
| Gene ID | 5788 |
| Uniprot ID | PTPRC_HUMAN |
| Accession Number | ENSG00000081237 |
| Immunogen | Purified recombinant peptide derived from within residues 1, 260 aa to the C-terminus of human CD45 produced in E. coli. |
| Immunogen Region | 1260aa C-Term |
| Immunogen Sequence | ANCVNPLGAPEKLPEAKEQA NCVNPLGAPEKLPEAKEQAE CVNPLGAPEKLPEAKEQAEG |
| Specificity | Using Jurkat cell lysate detects a band 180-220 kDa by Western blot. |
Additional Info
| Tissue Specificity | Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes. |
| Post Translational Modifications | Heavily N- and O-glycosylated. |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact.CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. |
| Protein Name | Receptor-Type Tyrosine-Protein Phosphatase CLeukocyte Common AntigenL-CaT200Cd Antigen Cd45 |
| Database Links | Reactome: R-HSA-202427Reactome: R-HSA-416700Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftSynapseColocalized With Dpp4 In Membrane Rafts |
| Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibodyAnti-Leukocyte Common Antigen antibodyAnti-L-Ca antibodyAnti-T200 antibodyAnti-Cd Antigen Cd45 antibodyAnti-PTPRC antibodyAnti-CD45 antibody |
Information sourced from Uniprot.org