• Anti-Rab27 pan antibody at 1:500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Anti-Rab27a antibody Immunohistochemistry staining of human mammary tissue immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval antibody concentration 1:200
  • Immunostaining of primary RPE cells with Rab27 antibody (1:50)
  • Anti-RAB27A antibody (120aa C-Term) (STJ140074)
  • Immunostaining of primary RPE cells with Rab27 antibody (1:50)
  • Immunostaining of B6-RPE07 cells with Rab27 antibody (1:50)

Anti-RAB27A antibody (120aa C-Term) (STJ140074)

SKU:
STJ140074

£85.50 - £532.50
Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/ELISA
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB27, member RAS oncogene family (120aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IHC-F 1:50-1:400
IHC-P 1:50-1:400
IF 1:50-1:250
ELISA ND
Ferreira JV Soares AR Ramalho J et al. Sci Adv 2022 Mar PMID: 35333565
Ferreira JV Rosa Soares A Ramalho JS et al. PLoS One 2019 Oct. PMID: 31613922
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB27A
Gene ID: 5873
Uniprot ID: RB27A_HUMAN
Immunogen Region: 120aa C-Term
Accession Number: ENSG00000069974
Specificity: Detects total Rab27 levels by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a and GFP-Rab27b cds.
Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a and rab27b produced in E. coli.
Tissue Specificity Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Protein Name Ras-Related Protein Rab-27a
Rab-27
Gtp-Binding Protein Ram
Database Links Reactome: R-HSA-264876
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Membrane
Lipid-Anchor
Melanosome
Late Endosome
Lysosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To Endosomal Exocytic Vesicles
Alternative Antibody Names Anti-Ras-Related Protein Rab-27a antibody
Anti-Rab-27 antibody
Anti-Gtp-Binding Protein Ram antibody
Anti-RAB27A antibody
Anti-RAB27 antibody

Information sourced from Uniprot.org

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