Rabbit Monoclonal Antibodies | Secondary Antibodies

Dose response curve of HRP conjugated Monkey IgA mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgA mAb in a 3 fold serial dilution from 1 Mu g/mL to 6. 97Ã—10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgA mAb has highly specific recognition of Monkey IgA while no or minimal cross reactivity to Monkey IgDã€Monkey IgEã€Monkey IgG3ã€Human IgAã€Mouse IgA.

Rabbit Anti-Cynomolgus monkey IgA antibody {HRP} (STJS001221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Cynomolgus monkey

£51.50 - £79.50

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Dose response curve of HRP conjugated Monkey IgD mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgD mAb in a 3 fold serial dilution from 1 Mu g/mL to 6. 97Ã—10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgD mAb has highly specific recognition of Monkey IgD while no or minimal cross reactivity to Monkey IgAã€Monkey IgEã€Monkey IgG3ã€Human IgDã€Mouse IgD.

Rabbit Anti-Cynomolgus monkey IgD antibody {HRP} (STJS001222)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Cynomolgus monkey

£51.50 - £79.50

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Rabbit Anti-Goat IgG Fab antibody {HRP} (STJS001261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Goat

£67.50 - £112.50

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Rabbit Anti-Goat IgG Fc antibody {HRP} (STJS001260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Goat

£67.50 - £112.50

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Rabbit Anti-Human IgA Fc antibody {HRP} (STJS001264)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£75.50 - £128.50

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Rabbit Anti-Human IgD antibody {HRP} (STJS001259)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Dose response curve of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1Ã—10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Human IgE (Fc) mAb has highly specific recognition of Human IgE while no or minimal cross reactivity to Human IgG1ã€Human IgG2ã€Human IgG3ã€Human IgG4ã€Human IgMã€Mouse IgGã€Rat IgG.

Rabbit Anti-Human IgE antibody {HRP} (STJS001218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Western blot analysis of various lysates, using HRP Rabbit anti-Human IgG1 (Fc) (STJS001216) at 1:4000-1:32000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Rabbit Anti-Human IgG1 Fc antibody {HRP} (STJS001216)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£67.50 - £112.50

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Dose response curve of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG2 (Fc) has highly specific recognition of Human IgG2ã€Human IgG while no or minimal cross reactivity to Human IgG1ã€Human IgG4ã€Human IgMã€Rat IgG.

Rabbit Anti-Human IgG2 Fc (99-326 aa) antibody {HRP} (STJS001227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Rabbit Anti-Human IgG4 antibody {HRP} (STJS001263)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£67.50 - £112.50

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Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0. 5 Mu g/mL to 3. 05Ã—10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1ã€Human IgG2ã€Human IgG3ã€Human IgG4ã€Mouse IgMã€Rat IgM.

Rabbit Anti-Human IgM antibody {HRP} (STJS001219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£46.50 - £68.50

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Rabbit Anti-Human Kappa Light Chain antibody {HRP} (STJS001265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£67.50 - £112.50

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Rabbit Anti-Monkey IgE Fc antibody {HRP} (STJS001262)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Monkey

£79.50 - £136.50

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Dose response curve of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgD (Fc) has highly specific recognition of Mouse IgD while no or minimal cross reactivity to Mouse IgG1ã€Mouse IgG2aã€Mouse IgG2bã€Mouse IgG2cã€Mouse IgG3ã€Mouse IgMã€Mouse IgEã€Mouse IgAã€Rat IgGã€Human IgG.

Rabbit Anti-Mouse IgD Fc (1-257 aa) antibody {HRP} (STJS001226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Mouse

£67.50 - £112.50

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Dose response curve of HRP conjugated Rabbit Anti-Mouse IgE mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgE mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Mouse IgE mAb has highly specific recognition of Mouse IgE while no or minimal cross reactivity to Mouse IgG1ã€Mouse IgG2aã€Mouse IgG2bã€Mouse IgG3ã€Mouse IgMã€Mouse IgAã€Human IgGã€Rat IgG.

Rabbit Anti-Mouse IgE Fc (50-388 aa) antibody {HRP} (STJS001223)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Mouse

£67.50 - £112.50

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Dose response curve of HRP conjugated Mouse IgG1 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG1 mAb in a 3 fold serial dilution from 1 Mu g/mL to 6. 97Ã—10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Mouse IgG1 mAb has highly specific recognition of Mouse IgG1 while no or minimal cross reactivity to Mouse IgG 2aã€Mouse IgG 2bã€Mouse IgG3ã€Mouse IgMã€Human IgG1ã€Rat IgG1.

Rabbit Anti-Mouse IgG1 antibody {HRP} (STJS001220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Mouse

£51.50 - £79.50

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Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate. , blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 Mu g/mL to 6. 1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1ã€Mouse IgG2aã€Mouse IgG2cã€Mouse IgG3ã€Mouse IgMã€Mouse IgEã€Mouse IgDã€Rat IgGã€Human IgG.

Rabbit Anti-Mouse IgG2b Fc (95-113 aa) antibody {HRP} (STJS001225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Mouse

£51.50 - £79.50

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Rabbit Anti-Mouse IgG3 Fc antibody {Unconjugated} (STJS001266)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse

£67.50 - £112.50

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Western blot analysis of lysates from Mouse IgM using HRP Rabbit anti-Mouse IgM mAb (STJS001217) at 1:50000 dilution. <br/>Lysates/proteins: 50ng per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Rabbit Anti-Mouse IgM Fc antibody {HRP} (STJS001217)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse

£201.50 - £379.50

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GFP antibody western blot 293T cells STJS001242

Rabbit Anti-Species-independent GFP Tag antibody {HRP} (STJS001242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species-independent

£187.50 - £821.50

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✓	Subclass and fragment specific Many clones in this range target a single immunoglobulin subclass (IgG1, IgG2, IgG3, IgG4, IgM, IgA, IgD or IgE) or a specific fragment (Fc or Fab), letting a subclass or fragment be detected without cross-reactivity to the others.
✓	Ready-to-use HRP conjugation 19 of the 20 products in this range are supplied HRP-conjugated, ready for direct use in colorimetric or chemiluminescent detection without a separate labelling step.
✓	Specificity data on the product page Product pages in this range include ELISA dose response data showing binding to the target subclass alongside minimal cross-reactivity to related subclasses, so specificity can be checked before ordering.
✓	Coverage across key research species The range spans immunoglobulin from human, mouse, cynomolgus monkey and goat, supporting subclass-level detection across the species most used in immunoassay development.

✓	Subclass-specific ELISA HRP-conjugated rabbit monoclonal secondaries are the main format used across this range for ELISA, detecting a specific immunoglobulin subclass captured or coated on the plate.
✓	Western blotting Several clones in this range are validated for western blot, detecting a primary antibody or immunoglobulin subclass bound to the membrane.
✓	Flow cytometry and IHC-P Selected clones in the range are also validated for flow cytometry or immunohistochemistry on paraffin sections, alongside their ELISA and western blot applications.

Rabbit Monoclonal Secondary Antibodies

Hybridoma-Based Production in a Rabbit Host

Why use rabbit monoclonal secondary antibodies?

Typical applications

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