St John's Laboratory Ltd

Western blot analysis of extracts of various cell lines, using (STJ119211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HMGCR antibody (783-791) (STJ119211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-NDFIP1 antibody (N-Term) (STJ13101435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£322.50

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Human FBXW7 protein (Recombinant) (N-His) (STJP006396)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-FBXW7 antibody (STJ11108359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SQSTM1 antibody (STJ195471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Thr269/Ser272 antibody (STJ196434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-SQSTM1 antibody (400-440) {Biotin} (STJ503126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-USP9X antibody (C-Term) (STJ73828)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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Western blot analysis of various lysates using USP22 Rabbit polyclonal antibody (STJ118728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-USP22 antibody (50-150) (STJ118728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ELOC antibody (40-120 aa) (STJ190401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human UBE2I/UBC9 protein (Recombinant) (N-His) (STJP005051)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human UBE2I/UBC9 protein (Recombinant) (N-GST) (STJP014577)

Host :

E.coli

Applications :

ELISA/WB

£454.50

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Immunofluorescence analysis of L929 cells using FBXW8 Rabbit polyclonal antibody (STJ11100092) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FBXW8 antibody (500-598) (STJ11100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human SPOP protein (Recombinant) (N-His) (STJP007348)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-SPOP antibody [PT1177R] (STJA0040212)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Western blot analysis of extracts of PC-3 cells, using SPOP Rabbit monoclonal antibody (STJ11103042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SPOP antibody (250-350) [S2MR] (STJ11103042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Recombinant-SPOP antibody [RM1E35] (STJA0023029)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£300.50 - £457.50

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Anti-FBXW7 antibody [PT1440R] (STJA0040302)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from HepG2 cells, using USP22 Rabbit mAb (STJ11102464) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-USP22 antibody (1-175) [S4MR] (STJ11102464)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using KBTBD7 antibody (STJ29529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-KBTBD7 antibody (475-684) (STJ29529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-MARCH-VII/Axotrophin antibody (Internal) (STJ71443)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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GAN Positive Control for STJ507836 peptide (STJ507838)

Applications :

WB

£308.50

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Human UBE2M protein (Recombinant) (N-His) (STJP005013)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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USP9X antibody western blot HeLa lysates STJA0036843

Anti-USP9X antibody [R09-5B7] (STJA0036843)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g SQSTM1/p62 antibody (STJ113162). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113162) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Recombinant-SQSTM1/p62 antibody [RM2E46] (STJA0025416)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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SQSTM1/p62 antibody immunocytochemistry U87-MG STJA0034886

Anti-SQSTM1/p62 antibody [R06-9J2] (STJA0034886)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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STJ70879 (0. 3µg/ml) staining of HEPG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Rabring 7/BCA2 antibody (Internal) (STJ70879)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Human CUL4B protein (Recombinant) (N-His) (STJP007349)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g RING1A antibody (STJ11103112). Western blot was performed from the immunoprecipitate using RING1A antibody (STJ11103112) at a dilution of 1:1000.

Anti-RING1A antibody (100-200) [S2MR] (STJ11103112)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunohistochemistry analysis of RING1A in paraffin-embedded mouse stomach using RING1A Rabbit polyclonal antibody (STJ11103202) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-RING1A antibody (207-406) (STJ11103202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using TRAF7 Rabbit polyclonal antibody (STJ118056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-TRAF7 antibody (260-400) (STJ118056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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RING1 antibody western blot Ramos lysates STJA0037108

Anti-RING1 antibody [R03-4V1] (STJA0037108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-FBXW7 antibody (STJA0007060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-Trim24-S1043 Rabbit mAb (STJ11105806) at1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Trim24-S1043 antibody [S5806RM] (STJ11105806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ110069) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (1-440) (STJ110069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Ser403 antibody (STJ196433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-SQSTM1 antibody (300-360 aa) (STJ13101972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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ICC staining HeLa cells using antibody autophagosome localization with DAPI nuclear stain STJA0040636

Anti-SQSTM1 antibody (Full-Length) (STJA0040636)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ICC/IHC/WB

Reactivity :

Human/Mouse/Rat

£294.50 - £703.50

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Anti-Sequestosome 1 antibody (C-Term) (STJA0040949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F

Reactivity :

Bovine/Human/Mouse/Rat

£528.50

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Anti-SQSTM1/p62 antibody [1F2] (STJA0032743)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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STJ73302 ( (2µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MARCH7/Axotrophin antibody (Internal) (STJ73302)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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Anti-GAN antibody (400-450) {FITC} (STJ507836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Human NOSIP (Nitric Oxide Synthase Interacting Protein) Sandwich ELISA Kit (STJE0011016)

Applications :

ELISA

Reactivity :

Human

£386.50 - £537.50

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Rat vHL (Von Hippel Lindau Tumor Suppressor) Sandwich ELISA Kit (STJE0013037)

Applications :

ELISA

Reactivity :

Rat

£451.50 - £629.50

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Human NOSIP (Nitric Oxide Synthase Interacting Protein) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017403)

Applications :

ELISA

Reactivity :

Human

£404.50 - £562.50

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Rat vHL (Von Hippel Lindau Tumor Suppressor) Sandwich ELISA Kit Ready-To-Use Kit (STJE0019427)

Applications :

ELISA

Reactivity :

Rat

£473.50 - £659.50

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Anti-Mitofusin 1 antibody (Internal) (STJ73826)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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