• Western blot analysis of various lysates using TRAF7 Rabbit polyclonal antibody (STJ118357) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-TRAF7 antibody (260-400) (STJ118357)

SKU:
STJ118357

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRAF7 (260-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRAF7
Gene ID: 84231
Uniprot ID: TRAF7_HUMAN
Immunogen Region: 260-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human TRAF7 (NP_115647.2).
Immunogen Sequence: PHSKYGCTFIGNQDTYETHL ETCRFEGLKEFLQQTDDRFH EMHVALAQKDQEIAFLRSML GKLSEKIDQLEKSLELKFDV LDENQSKLSEDLMEFRRDAS MLNDELSHINARLNMGILGS YDPQQIFKCKGTFVGHQGPV W
Tissue Specificity Ubiquitously expressed with high levels in skeletal muscle, heart, colon, spleen, kidney, liver and placenta.
Post Translational Modifications Phosphorylated by MAP3K3. Ubiquitinates itself upon phosphorylation.
Function E3 ubiquitin and SUMO-protein ligase that plays a role in different biological processes such as innate immunity, inflammation or apoptosis. Potentiates MAP3K3-mediated activation of JUN/AP1 and DDIT3 transcriptional regulators. Negatively regulates MYB transcriptional activity by sequestering it to the cytosol via SUMOylation. Plays a role in the phosphorylation of MAPK1 and/or MAPK3, probably via its interaction with MAP3K3. Negatively regulates RLR-mediated innate immunity by promoting 'Lys-48'-linked ubiquitination of TBK1 through its RING domain to inhibit the cellular antiviral response. Promotes 'Lys-29'-linked polyubiquitination of NEMO/IKBKG and RELA leading to targeting these two proteins to lysosomal degradative pathways, reducing the transcriptional activity of NF-kappa-B.
Protein Name E3 Ubiquitin-Protein Ligase Traf7
Ring Finger And Wd Repeat-Containing Protein 1
Ring Finger Protein 119
Ring-Type E3 Ubiquitin Transferase Traf7
Tnf Receptor-Associated Factor 7
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasmic Vesicle
Cytoplasm
Nucleus
Colocalizes With Map3k3 To Vesicle-Like Structures Throughout The Cytoplasm
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Traf7 antibody
Anti-Ring Finger And Wd Repeat-Containing Protein 1 antibody
Anti-Ring Finger Protein 119 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Traf7 antibody
Anti-Tnf Receptor-Associated Factor 7 antibody
Anti-TRAF7 antibody
Anti-RFWD1 antibody
Anti-RNF119 antibody

Information sourced from Uniprot.org

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