• Western blot analysis of extracts of various cell lines, using (STJ119211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using HMGCR antibody (STJ119211) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal immunofluorescence analysis of NIH-3T3 cells using HMGCR Polyclonal Antibody (STJ119211) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using HMGCR rabbit polyclonal antibody (STJ119211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using HMGCR rabbit polyclonal antibody (STJ119211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using HMGCR rabbit polyclonal antibody (STJ119211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMGCR antibody (783-791) (STJ119211)

SKU:
STJ119211

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGCR (783-791) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGCR
Gene ID: 3156
Uniprot ID: HMDH_HUMAN
Immunogen Region: 783-791
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 783-791 of human HMGCR (NP_000850.1).
Immunogen Sequence: ASGPTNEDL
Tissue Specificity Isoform 1: Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland. Isoform 2: Detected in the cerebellum, fetal brain, testis and adrenal gland. Isoform 3: Low abundance except in skin, esophagus, and uterine cervix.
Post Translational Modifications N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2. The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity.
Function Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs.
Protein Name 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase
Hmg-Coa Reductase
Database Links Reactome: R-HSA-191273
Reactome: R-HSA-1989781 P04035-1
Reactome: R-HSA-2426168 P04035-1
Reactome: R-HSA-9619665
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Peroxisome Membrane
Alternative Antibody Names Anti-3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase antibody
Anti-Hmg-Coa Reductase antibody
Anti-HMGCR antibody

Information sourced from Uniprot.org

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