St John's Laboratory Ltd

Western blot analysis of various lysates, using Chk1 Rabbit polyclonal antibody (STJ11104296) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q60s.

Anti-CHEK1 antibody (148-307) (STJ11104296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-Phospho-Chk1-Ser345 antibody [PT0651R] (STJA0039191)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Human CHEK1 protein (Recombinant) (N-His) (STJP015221)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Phospho-Chk1 S296 antibody western blot HEK293 lysates STJA0036032

Anti-Phospho-Chk1-Ser296 antibody [R02-4Z6] (STJA0036032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of PC-12 cells using Chk1 Rabbit polyclonal antibody (STJ29967) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-CHEK1 antibody (148-307) (STJ29967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Chk1 antibody [PT0606R] (STJA0039379)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Chk1 antibody western blot K562 lysates STJA0034168

Anti-Chk1 antibody [R07-4K2] (STJA0034168)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot Jurkat STJA0036230

Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Chk1 S280 antibody western blot 293T Calyculin A STJA0035702

Anti-Phospho-Chk1-Ser280 antibody [R32-4D-1] (STJA0035702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Chk1 antibody western blot THP-1 3T3 PC-12 STJA0035707

Anti-Chk1 antibody [R02-1D6] (STJA0035707)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Biotinylated STJ70109 (2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-SIAH1, Biotinylated antibody (N-Term) {Biotin} (STJ73231)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-Chk1-S317 Rabbit pAb (STJ22072). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CHEK1-S317 antibody (STJ22072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S280) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser280 antibody (251-300 aa) (STJ90221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser286 antibody (256-305 aa) (STJ90705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of MCF7 cells using Chk1 Polyclonal Antibody

Anti-CHEK1 antibody (286-335 aa) (STJ92267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Chk1 monoclonal antibody against A431 (1) , HeLa (2) , NIH/3T3 (3) and K562 (4) cell lysate.

Anti-CHEK1 antibody [2G1D5] (STJ97955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit monoclonal antibody (STJ11104149) at1:2000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-CHEK1 antibody [S4149RM] (STJ11104149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit monoclonal antibody (STJ11105894) at 1:2000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Phospho-CHEK1-S280 antibody [S5894RM] (STJ11105894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Histone H2AX antibody immunocytochemistry HeLa cells STJA0035222

Anti-Histone H2A.X antibody [R02-3C8] (STJA0035222)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Timeless Positive Control for STJ503335 peptide (STJ505218)

Applications :

WB

£308.50

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Anti-SIAH1 antibody [SRM14616] (STJ11109346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (STJ11102618) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CHEK1-S296 antibody [S8MR] (STJ11102618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S317) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser317 antibody (286-335 aa) (STJ90222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-CHEK1 antibody [RM1D26] (STJA0022921)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (STJ22071) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CHEK1-S280 antibody (STJ22071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of 293T cells using Phospho-Chk1 (S301) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CHEK1-Ser301 antibody (271-320 aa) (STJ90706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-Histone H2AX antibody western blot HepG2 STJA0034346

Anti-Phospho-Histone H2A.X-Ser139 antibody [R09-4A7] (STJA0034346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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STJ70109 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SIAH1 antibody (N-Term) (STJ70109)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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SDS-PAGE gel of recombinant human SIAH1 protein 3µg under reducing conditions Coomassie blue stain

Human SIAH1 protein (Recombinant) (His-Tag) (STJP017778)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb (STJ27513) at 1:400 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-Histone H2A-S139 antibody (STJ27513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser296 antibody (266-315 aa) (STJ90849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit monoclonal antibody (STJ11105881) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CHEK1-S280 antibody [S5881RM] (STJ11105881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-Chk1-Ser317 antibody [PT0744R] (STJA0040059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Phospho-Histone H2AX antibody western blot 3T3 cells STJA0033230

Anti-Phospho-Histone H2A.X-Ser139 antibody [2A9-B5] (STJA0033230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX Ser139 antibody western blot 3T3 STJA0033231

Anti-Phospho-Histone H2A.X-Ser139 antibody [7G9-H4] (STJA0033231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SIAH1 antibody (157-207 aa) (STJ194578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit pAb (STJ29344) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CHEK1-S345 antibody (STJ29344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser345 antibody (310-359 aa) (STJ90223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-H2AX-Ser139 antibody (STJA0009882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Chk1 antibody [SRM13609] (STJ11108339)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Histone H2AX antibody immunocytochemistry HL-60 cells STJA0034412

Anti-Histone H2A.X antibody [R02-5E6] (STJA0034412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry of paraffin embedded mouse brain using RAD51 at dilution of 1:800

Anti-Rad51 antibody [SM5108] (STJA0005108)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse/Rat

£153.50 - £228.50

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Human DDB2 protein (Recombinant) (N-His) (STJP005771)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Western blot analysis of extracts of various cell lines, using Rad51 rabbit monoclonal antibody (STJ11101833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RAD51 antibody (1-100) [S3MR] (STJ11101833)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BRCA1 (Phospho-Ser1423) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BRCA1-Ser1423 antibody (1391-1440 aa) (STJ90198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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