• Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SIAH1 antibody (157-207 aa) (STJ194578)

SKU:
STJ194578

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase SIAH1 (157-207 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIAH1
Gene ID: 6477
Uniprot ID: SIAH1_HUMAN
Immunogen Region: 157-207 aa
Specificity: This antibody detects endogenous levels of SIAH1 at Human/Rat
Immunogen: Synthesized peptide derived from the human SIAH1 at the amino acid range 157-207
Post Translational Modifications Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4. Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1.
Protein Name E3 Ubiquitin-Protein Ligase Siah1
Ring-Type E3 Ubiquitin Transferase Siah1
Seven In Absentia Homolog 1
Siah-1
Siah-1a
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-977225
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Partially Nuclear
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Siah1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Siah1 antibody
Anti-Seven In Absentia Homolog 1 antibody
Anti-Siah-1 antibody
Anti-Siah-1a antibody
Anti-SIAH1 antibody
Anti-HUMSIAH antibody

Information sourced from Uniprot.org

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