Vesicle-Mediated Transport | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TUBB3 antibody [M7] (STJ97037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TUBB4A antibody (218-268 aa) (STJ192957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunocytochemistry analysis of beta IV Tubulin (green) in Hela using beta IV Tubulin antibody, and DAPI (blue)

Anti-TUBB4A antibody (430-444 aa) [R06-6A5] (STJA0011034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of U2OS cells using TUBB8 antibody (STJ11102746) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TUBB8 antibody (344-444 aa) (STJ11102746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various lysates using TUBB8 Rabbit pAb (STJ11105740) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TUBB8 antibody (350-444) (STJ11105740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-Tuberin antibody [R04-1I3] (STJA0035390)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£140.50 - £308.50

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Anti-Tuberin antibody [R06-0D3] (STJA0035391)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Tubulin Alpha antibody [TU-02] (STJ16100478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC

Reactivity :

Human/Mouse/Pig/PlantS./Rat

£331.50 - £1,476.50

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Immunofluorescence analysis of U-2 OS cells using Tubulin beta-1 chain Rabbit monoclonal antibody (STJ11103147) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Tubulin beta-1 chain antibody (352-451) [S7MR] (STJ11103147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Dog/Monkey

£118.50 - £521.50

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Anti-TUG antibody [R34-2C-3] (STJA0035545)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of various lysates, using TUG/ASPSCR1 antibody (STJ11102758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TUG/ASPSCR1 antibody (200-300) [S8MR] (STJ11102758)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of MCF-7 cells using TXNDC5 Rabbit polyclonal antibody (STJ116087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TXNDC5 antibody (1-324) (STJ116087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TXNDC5 antibody (STJ192123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-TXNDC5 antibody [100] (STJA0032731)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Monkey/Mouse/Rat

£224.50 - £308.50

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Immunocytochemistry analysis of Ubiquitin 60S Ribosomal Protein L40 (green) in Hela using Ubiquitin 60S Ribosomal Protein L40 antibody, and DAPI (blue).

Anti-UBA52 antibody (39-52 aa) [R05-5D6] (STJA0012632)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-UBA52 antibody (71-120 aa) (STJ95481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Ub antibody at 2500 dilution; cells stimulated with MG132; lysate at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-UBA52 antibody (STJ140085)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Anti-UBA52 antibody [PT0081R] (STJA0039231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-UBA52 antibody [SRM13090] (STJ11107820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-UBAP1 antibody (STJ191588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Confocal imaging of MCF7 cells using K48-linkage Specific Ubiquitin Rabbit monoclonal antibody (STJ11101909, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-UBB antibody (1-100) [S9MR] (STJ11101909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (STJ11101766) at1:1000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-228) [S6MR] (STJ11101766)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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Immunofluorescence analysis of HeLa cells using Ubiquitin Rabbit polyclonal antibody (STJ11100145) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBB antibody (1-229) (STJ11100145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£110.50 - £505.50

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Western blot analysis of lysates from C6 cells using Ubiquitin Rabbit polyclonal antibody (STJ26009) at 1:900 dilution. C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UBB antibody (1-229) (STJ26009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HepG2 cells using UBC Rabbit polyclonal antibody (STJ29897) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-UBC antibody (1-100) (STJ29897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UBC antibody (160-210 aa) (STJ194141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-UBC/polyUb/Polyubiquitin-C antibody [SAA0328] (STJA0019255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Ubiquilin 2 antibody [6H9] (STJA0040772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/WB

Reactivity :

Human/Mouse

£528.50

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Anti-Ubiquitin antibody (STJA0040904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Ubiquitin antibody [1B8-8E7-1H7] (STJA0032907)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

£177.50 - £261.50

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Anti-Ubiquitin antibody [7F2-4B1-10D3] (STJA0032914)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Transfected

£133.50 - £281.50

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Anti-Ubiquitin antibody [R01-7F1] (STJA0034973)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Ubiquitin antibody [R02-4I6] (STJA0034974)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Anti-Ubiquitin antibody [SRM13312] (STJ11108042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Ubiquitin K48 antibody [R06-2H7] (STJA0034497)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Ubiquitin K63 antibody [R06-2A6] (STJA0036235)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Ubiquitin K63 antibody [R06-2H7] (STJA0035398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-UBQLN1 antibody (302-352) (STJA0006627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using UBQLN1 Rabbit polyclonal antibody (STJ117634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-UBQLN1 antibody (480-540) (STJ117634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using UBQLN2 Rabbit pAb (STJ111746) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-UBQLN2 antibody (1-150) (STJ111746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-UBQLN2 antibody (STJ191566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot with Anti-ULK1 Antibody (STJ503461) on ULK1 peptide. Antibody at 1:250 dilution in buffer.

Anti-ULK1 antibody (1000-1050) (STJ503461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/IP/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Panda

£400.50

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Anti-ULK1 antibody (1000-1050) {Biotin} (STJ503462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Panda

£497.50

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Immunostaining with Anti-ULK1 Antibody (FITC) (STJ503463) on human pDCs.

Anti-ULK1 antibody (1000-1050) {FITC} (STJ503463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/IP/WB

Reactivity :

Bovine/Human/Monkey/Mouse/Panda

£497.50

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Anti-ULK1 antibody (350-410 aa) (STJ13102584)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-ULK1 antibody (350-410 aa) (STJ13102585)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-ULK1 antibody (350-410 aa) (STJ13102589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Western blot analysis of lysates from Rat brain, using ULK1 Rabbit pAb (STJ111271) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-ULk1 antibody (350-450) (STJ111271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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