Anti-Ubiquilin 2 antibody [6H9] (STJA0040772)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant human ubiquilin 2 expressed and purified from E. coli.
General Information
| Short Description | Mouse monoclonal anti-Ubiquilin 2 for use in ICC and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 6H9 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:1000-1:2000ICC 1:500-1:1000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Aliquot and store at-20°C for up to six months after date of receipt. Avoid |
Target Information
| Gene Symbol | UBQLN2 |
| Gene ID | 29978 |
| Uniprot ID | UBQL2_HUMAN |
| Immunogen | Recombinant human ubiquilin 2 expressed and purified from E. coli. |
| Immunogen Sequence | Human |
| Specificity | In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all tranduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in cells transduced wit |
Additional Info
| Post Translational Modifications | Degraded during macroautophagy. |
| Function | Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Involved in the regulation of macroautophagy and autophagosome formation.required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs). |
| Protein Name | Ubiquilin-2Chap1Dsk2 HomologProtein Linking Iap With Cytoskeleton 2Plic-2Hplic-2Ubiquitin-Like Product Chap1/Dsk2 |
| Database Links | Reactome: R-HSA-8856825 |
| Cellular Localisation | CytoplasmNucleusMembraneCytoplasmic VesicleAutophagosomeColocalizes With A Subset Of ProteasomesNamely Those That Are Cytoskeleton Associated Or Free In The CytosolAssociated With Fibers In Mitotic Cells |
| Alternative Antibody Names | Anti-Ubiquilin-2 antibodyAnti-Chap1 antibodyAnti-Dsk2 Homolog antibodyAnti-Protein Linking Iap With Cytoskeleton 2 antibodyAnti-Plic-2 antibodyAnti-Hplic-2 antibodyAnti-Ubiquitin-Like Product Chap1/Dsk2 antibodyAnti-UBQLN2 antibodyAnti-N4BP4 antibodyAnti-PLIC2 antibodyAnti-HRIHFB2157 antibody |
Information sourced from Uniprot.org