Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from A-549 cells, using CCL2/MCP-1 Rabbit monoclonal antibody (STJ11104938) at 1:8000 dilution. A-549 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CCL2 antibody (22-99) [S4938RM] (STJ11104938)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Western blot analysis of A-549, using CCL2/MCP-1 antibody (STJ29416) at 1:2000 dilution. A-549 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-CCL2 antibody (24-99) (STJ29416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL2 antibody (STJ194973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis using MCP-1 monoclonal antibody against truncated MCP-1 recombinant protein.

Anti-CCL2 antibody [1A7B8] (STJ98233)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-CCL2/MCP-1 antibody [11k2] (STJA0027579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Human

$351.21 - $522.33

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Anti-CCL2/MCP-1 antibody [11k2] {APC} (STJA0029156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$428.49 - $652.05

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Anti-CCL2/MCP-1 antibody [11k2] {FITC} (STJA0028190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$351.21 - $522.33

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Anti-CCL2/MCP-1 antibody [11k2] {PerCP} (STJA0030122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$403.65 - $607.89

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Anti-CCL2/MCP-1 antibody [11k2] {PE} (STJA0031088)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$428.49 - $652.05

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Anti-CCL2/MCP-1 antibody [15D7] (STJA0019349)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA

Reactivity :

Human

$471.27 - $1,997.55

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Anti-CCL2/MCP-1 antibody [SRM14620] (STJ11109350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-CCL2/MCP-1 nanobody [SAA1269] (STJN000140)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/RIA

Reactivity :

Human

$522.33 - $2,252.85

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-CCL20 antibody (31-80 aa) (STJ98775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-CCL20/MIP-3-alpha antibody [SAA0277] (STJA0019062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Human

$471.27 - $1,997.55

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Immunohistochemistry analysis of CCL21 in paraffin-embedded human stomach using CCL21 Rabbit polyclonal antibody (STJ22930) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CCL21 antibody (24-134) (STJ22930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:200

Anti-CCL21 antibody (71-120 aa) (STJ98793)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CCL21/6Ckine antibody [SAA1498] (STJA0019926)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$471.27 - $1,997.55

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Anti-CCL22 antibody (1-50) (STJA0010198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded Human spleen using CCL22 Rabbit pAb (STJ22931) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-CCL22 antibody (25-93) (STJ22931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

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Anti-CCL22 antibody (31-80) (STJA0010152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CCL25, using CCL25 rabbit polyclonal antibody (STJ116184) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-CCL25 antibody (24-150) (STJ116184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL25 antibody (30-110 aa) (STJ191425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-CCL25 antibody [SRM14927] (STJ11109657)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using CCL27 Rabbit pAb (STJ111906) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CCL27 antibody (25-112) (STJ111906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of mouse-kidney lysis using CCL27 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-CCL27 antibody (51-100 aa) (STJ97677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates, using CCL28 Rabbit polyclonal antibody (STJ22934) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CCL28 antibody (20-127) (STJ22934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CCL28 antibody (50-130 aa) (STJ191426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of recombinant Human CCL3/MIP-1 alpha Protein using CCL3 Rabbit polyclonal antibody (STJ11105636) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 5 ng/2ng/1ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 10s.

Anti-CCL3 antibody (23-92) (STJ11105636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of lysates from THP-1 cells, using CCL3 Rabbit polyclonal antibody (STJ29705) at 1:1000 dilution. THP-1 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CCL3 antibody (24-92) (STJ29705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of MIP-1 Alpha antibody in paraffin-embedded human lung carcinoma tissue.

Anti-CCL3 antibody (26-75 aa) (STJ94130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CCL3/MIP-1-alpha antibody [SAA0454] (STJA0027631)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CCL3/MIP-1-alpha antibody [SAA0454] {APC} (STJA0029116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Anti-CCL3/MIP-1-alpha antibody [SAA0454] {FITC} (STJA0028150)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CCL3/MIP-1-alpha antibody [SAA0454] {PerCP} (STJA0030082)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$366.39 - $547.17

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Anti-CCL3/MIP-1-alpha antibody [SAA0454] {PE} (STJA0031048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Anti-CCL3/MIP-1-alpha nanobody [SAA1270] (STJN000132)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/RIA

Reactivity :

Human

$522.33 - $2,252.85

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Anti-CCL3/MIP-alpha antibody [SRM13523] (STJ11108253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-CCL3/MIP-alpha antibody [SRM13524] (STJ11108254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of extracts of B-cell cells, using CCL3L1 antibody (STJ22935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CCL3L1 antibody (24-93) (STJ22935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using CCL4 antibody (STJ22936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CCL4 antibody (1-92) (STJ22936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$152.49 - $697.59

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Western blot analysis of HL-60, 22RV-1 cells using MIP-1 Beta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CCL4 antibody (41-90 aa) (STJ96572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CCL4/MIP-1 beta antibody [SRM13555] (STJ11108285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$0.00

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Anti-CCL4/MIP-1-beta antibody [SAA0455] (STJA0027632)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CCL4/MIP-1-beta antibody [SAA0455] {APC} (STJA0029155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Anti-CCL4/MIP-1-beta antibody [SAA0455] {FITC} (STJA0028189)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $471.27

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Anti-CCL4/MIP-1-beta antibody [SAA0455] {PerCP} (STJA0030121)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$366.39 - $547.17

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Anti-CCL4/MIP-1-beta antibody [SAA0455] {PE} (STJA0031087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $584.43

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Western blot analysis of K562 cells using MIP-1b Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-CCL4L1/CCL4L2 antibody (31-80 aa) (STJ97347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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