• Western blot analysis of extracts of various cell lines, using B-Raf antibody (STJ22828) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BRAF antibody (153-313) (STJ22828)

SKU:
STJ22828

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRAF (153-313) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRAF
Gene ID: 673
Uniprot ID: BRAF_HUMAN
Immunogen Region: 153-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-313 of human B-Raf (NP_004324.2).
Immunogen Sequence: QKPIVRVFLPNKQRTVVPAR CGVTVRDSLKKALMMRGLIP ECCAVYRIQDGEKKPIGWDT DISWLTGEELHVEVLENVPL TTHNFVRKTFFTLAFCDFCR KLLFQGFRCQTCGYKFHQRC STEVPLMCVNYDQLDLLFVS KFFEHHPIPQEEASLAETAL T
Tissue Specificity Brain and testis.
Post Translational Modifications Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.
Protein Name Serine/Threonine-Protein Kinase B-Raf
Proto-Oncogene B-Raf
P94
V-Raf Murine Sarcoma Viral Oncogene Homolog B1
Database Links Reactome: R-HSA-1295596
Reactome: R-HSA-170968
Reactome: R-HSA-170984
Reactome: R-HSA-187706
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Colocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase B-Raf antibody
Anti-Proto-Oncogene B-Raf antibody
Anti-P94 antibody
Anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibody
Anti-BRAF antibody
Anti-BRAF1 antibody
Anti-RAFB1 antibody

Information sourced from Uniprot.org

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