Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (STJ11102561) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H2A-S139 antibody [S1MR] (STJ11102561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Phospho-Histone H2AX antibody western blot 3T3 cells STJA0033230

Anti-Phospho-Histone H2A.X-Ser139 antibody [2A9-B5] (STJA0033230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX Ser139 antibody western blot 3T3 STJA0033231

Anti-Phospho-Histone H2A.X-Ser139 antibody [7G9-H4] (STJA0033231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot Jurkat STJA0036230

Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot HepG2 STJA0034346

Anti-Phospho-Histone H2A.X-Ser139 antibody [R09-4A7] (STJA0034346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot Histone H2B antibody detection in Jurkat cells with phosphatase treatment STJA0041244

Anti-Phospho-Histone H2B-Ser-36 antibody (STJA0041244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemistry of baboon spleen with Anti-Phospho-Histone H3 primary antibody (S10) (STJ502483) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-Phospho-Histone H3 antibody (1-50) (STJ502483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£497.50

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Anti-Phospho-Histone H3 antibody (1-50) {Biotin} (STJ502484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£551.50

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Anti-Phospho-Histone H3 antibody (1-50) {FITC} (STJ502485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£551.50

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Phospho-Histone H3 antibody immunohistochemistry gastric cancer STJA0037643

Anti-Phospho-Histone H3-Ser10 antibody [BP6092] (STJA0037643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£244.50 - £335.50

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Phospho-Histone H3 antibody western blot HeLa STJA0035817

Anti-Phospho-Histone H3-Ser10 antibody [R06-6O9] (STJA0035817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 antibody immunocytochemistry HeLa cells STJA0035070

Anti-Phospho-Histone H3-Ser10/Thr11 antibody [R08-6C4] (STJA0035070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 Ser28 antibody western blot 3T3 HeLa STJA0034415

Anti-Phospho-Histone H3-Ser28 antibody [R03-7K5] (STJA0034415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Histone H3-T11 antibody [N123/19] (STJA0019175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£195.50 - £1,319.50

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Phospho-Histone H3 Thr3 antibody western blot C6 3T3 HeLa STJA0033904

Anti-Phospho-Histone H3-Thr3 antibody [R08-9D6] (STJA0033904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Histone H3 Thr3 antibody western blot HeLa STJA0035656

Anti-Phospho-Histone H3-Thr3 antibody [R21-3K-2] (STJA0035656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Histone H3 (Phospho-Thr3) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Histone H3.1-Thr3 antibody (1-50 aa) (STJ90291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Histone H3.3-S31 antibody [SRM13685] (STJ11108415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (STJ11101036) at 1:1000 dilution or Histone H4 Rabbit pAb (STJ29382). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-Histone H4-S1 antibody (STJ11101036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-Histone H4-Ser1 antibody (STJ197451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C overnight STJ91343

Anti-Phospho-Histone H4-Ser47 antibody (13-62 aa) (STJ91343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/IHC/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Histone H4-Thr80 antibody (STJ197450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot Histone H4 Tyr-72 phosphorylation in treated and untreated PC3 cells STJA0041245

Anti-Phospho-Histone H4-Tyr-72 antibody (STJA0041245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Western blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HNRNPC-Ser260 antibody (231-280 aa) (STJ90910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HUVEC cells, using Histamine H1 Receptor (Phospho-Ser398) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-HRH1-Ser398 antibody (364-413 aa) (STJ91347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Phospho-Hsp27 S78 antibody western blot A431 lysates STJA0036207

Anti-Phospho-Hsp27-Ser78 antibody [R01-8F1] (STJA0036207)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-Hsp27 antibody western blot HeLa heat shock STJA0036052

Anti-Phospho-Hsp27-Ser82 antibody [R04-3X1] (STJA0036052)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-HSP27/HSPB1-S15 antibody [SRM13690] (STJ11108420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-HSP90AA1-Thr5/7 antibody (STJ196324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, HSP90 Beta (phospho Ser226) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-HSP90AB1-Ser226 antibody (201-250 aa) (STJ90725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP90 Beta (S254) Polyclonal Antibody

Anti-Phospho-HSP90AB1-Ser254 antibody (226-275 aa) (STJ91017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (STJ22178) at 1:600 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-HSPB1-S15 antibody (STJ22178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit pAb (STJ22181) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by serum-starvation overnight or treated by Anisomycin (25 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-HSPB1-S78 antibody (STJ22181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (STJ11102612) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S78 antibody [S2MR] (STJ11102612)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-HSPB1-S78/82 antibody (STJ194697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit pAb (STJ22179) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-HSPB1-S82 antibody (STJ22179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (STJ11102611) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-HSPB1-S82 antibody [S1MR] (STJ11102611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-HSP27 (S15) Polyclonal Antibody

Anti-Phospho-HSPB1-Ser15 antibody (5-54 aa) (STJ90294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP27 (S78) Polyclonal Antibody STJ90295

Anti-Phospho-HSPB1-Ser78 antibody (45-94 aa) (STJ90295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-HSP27 (S82) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-HSPB1-Ser82 antibody (48-97 aa) (STJ90296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-I Kappa B Alpha-Ser-32/Ser-36 antibody [39A1413] (STJA0041251)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-Phospho-I Kappa B Alpha-Tyr-305 antibody (STJA0041254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-I Kappa B Alpha-Tyr-42 antibody (STJA0041253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IP

Reactivity :

Human/Mouse/Rat

£542.50

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Phospho-IGF1R antibody western blot C6 3T3 HeLa STJA0034476

Anti-Phospho-IGF1 Receptor-Tyr1166 antibody [R06-6B1] (STJA0034476)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-IGF1R-Tyr1131 antibody (STJ196325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-IGF1R-Tyr1135 antibody (STJ196326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-IGF1R-Tyr1161 antibody (STJ99596)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody diluted at 1：500

Anti-Phospho-IGF1R-Tyr1165/Y1166 antibody (1131-1180 aa) (STJ90299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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