Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various cells using Strad Polyclonal Antibody

Anti-STRADA antibody (11-60 aa) (STJ95829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Mouse skeletal muscle, using STRADA Rabbit polyclonal antibody (STJ11104490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-STRADA antibody (25-210) (STJ11104490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts of mouse skeletal muscle, using STRADA antibody (STJ112374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-STRADA antibody (25-210) (STJ112374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-STRADA antibody (25-210) (STJ119985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-STRADA antibody (381-431) (STJ503156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-STRADA antibody (381-431) {Biotin} (STJ503159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-STRADA antibody (381-431) {FITC} (STJ503160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£528.50

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Immunohistochemistry of baboon kidney with Anti-STRADA primary antibody (STJ503155) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-STRADA antibody (50-100) (STJ503155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-STRADA antibody (50-100) {Biotin} (STJ503157)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-STRADA antibody (50-100) {FITC} (STJ503158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Western blot analysis of various lysates using STRADB Rabbit pAb (STJ111364) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-STRADB antibody (209-418) (STJ111364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-STRADB antibody (313-363 aa) (STJ194305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and STRAP knockout (KO) 293T cells, using [KO Validated] STRAP Rabbit polyclonal antibody (STJ11100865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-STRAP antibody (1-350) (STJ11100865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g STRAP antibody (STJ11104299). Western blot was performed from the immunoprecipitate using STRAP antibody (STJ11104299) at a dilution of 1:1000.

Anti-STRAP antibody (1-350) (STJ11104299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using STRAP antibody (STJ27760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-STRAP antibody (1-350) (STJ27760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-STRAP antibody (STJA0006955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human liver damage using STRN antibody (STJ110045) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-STRN antibody (581-780) (STJ110045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from K562 cells, using Striatin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-STRN antibody (C-Term) (STJ96457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g STUB1 antibody (STJ113341). Western blot was performed from the immunoprecipitate using STUB1 antibody (STJ113341) at a dilution of 1:1000.

Anti-STUB1 antibody (1-303) (STJ113341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-STUB1 antibody (160-240 aa) (STJ192459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-STUB1 antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-STUB1 antibody (STJ140143)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-STX5 antibody (233-283 aa) (STJ193534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from rat brain, using STX5 Rabbit pAb (STJ110753) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-STX5 antibody (55-355) (STJ110753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunofluorescence analysis of SH-SY5Y cells using [KO Validated] Substance P Rabbit polyclonal antibody (STJ11103444) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Substance P antibody (1-100) (STJ11103444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from HUVEC and MCF-7 cells, using SUCNR1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-SUCNR1 antibody (100-149 aa) (STJ93400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Immunofluorescence analysis of MCF-7 cells using SUFU Rabbit polyclonal antibody (STJ115390). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SUFU antibody (325-484) (STJ115390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SUFU antibody (STJ28840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SUFU antibody (325-484) (STJ28840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SUFU antibody (STJA0007244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g SUZ12 antibody (STJ110096). Western blot was performed from the immunoprecipitate using SUZ12 antibody (STJ110096) at a dilution of 1:500.

Anti-SUZ12 antibody (470-739) (STJ110096)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis using SUZ12 monoclonal antibody against HEK293 (1) and SUZ12-hIgGFc transfected HEK293 (2) cell lysate.

Anti-SUZ12 antibody [2C11] (STJ98404)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of L929 cells using SWAP70 Rabbit polyclonal antibody (STJ117057) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SWAP70 antibody (316-585) (STJ117057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot detection of SWAP70 in COS7, 293T, C6, 3T3 and Hela cell lysates and using SWAP70 mouse mAb (1:1000 diluted).Predicted band size: 70KDa.Observed band size: 70KDa.

Anti-SWAP70 antibody [2E9-E8-E9] (STJ99160)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunohistochemistry of mouse tissue with Anti-SYDE1 primary antibody (STJ503179) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SYDE1 antibody (680-735) (STJ503179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse

£427.50

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Anti-SYDE1 antibody (680-735) {Biotin} (STJ503180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse

£528.50

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Anti-SYDE1 antibody (680-735) {FITC} (STJ503181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse

£528.50

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Western blot with Anti-SYK Antibody (STJ503182) on mouse bladder. Antibody at 1:500 dilution in buffer.

Anti-SYK antibody (100-150) (STJ503182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SYK antibody (100-150) {Biotin} (STJ503183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SYK antibody (100-150) {FITC} (STJ503184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of U2OS using Syk antibody (STJ25758) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYK antibody (250-350) (STJ25758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SYK antibody (290-370 aa) (STJ95845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SYK antibody (Central region) [M373] (STJA0005609)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-SYK antibody (STJ99608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Syk antibody [SYK-01] (STJ16100208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P/ICC

Reactivity :

Human/Rat/Mouse

£537.50

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Western blot analysis of extracts of various cell lines, using SynGAP Rabbit monoclonal antibody (STJ11103043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SynGAP antibody (1244-1343) [S3MR] (STJ11103043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of SynGAP in rat Brain lysates using SynGAP antibody.

Anti-SYNGAP1 antibody (1330-1343 aa) [R03-4F4] (STJA0010747)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat

£264.50

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Anti-SYNGAP1 antibody (STJ192152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Syntaxin 5 antibody (STJ13100348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Anti-Syntaxin-5 antibody (STJ13100347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human/Marmoset

£331.50

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