Signal Transduction | Research Area | St John's Laboratory

Immunofluorescence analysis of HeLa cells using PKC alpha Rabbit monoclonal antibody (STJ11105054) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKCA antibody [S5054RM] (STJ11105054)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunocytochemistry analysis of PKC beta 2 (green) in K562 using PKC beta 2 antibody, and DAPI (blue).

Anti-PRKCB antibody (170-350 aa) [R06-0C2] (STJA0012652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Immunofluorescence analysis of NIH/3T3 cells using PKC-beta Rabbit polyclonal antibody (STJ29922) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKCB antibody (220-673) (STJ29922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using PKC-beta Rabbit polyclonal antibody (STJ115587) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKCB antibody (260-340) (STJ115587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells, using PKCB Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PRKCB antibody (622-671 aa) (STJ95121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using PKC delta Rabbit polyclonal antibody (STJ29804) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PRKCD antibody (1-160) (STJ29804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using PKC delta Rabbit monoclonal antibody (STJ11102340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKCD antibody (547-653) [S0MR] (STJ11102340)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PRKCD antibody (612-661 aa) (STJ95126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-PRKCD antibody (N-Term) [M242] (STJA0005534)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£394.50

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Immunohistochemistry of mouse brain with Anti-PRKCE primary antibody (STJ502516) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-PRKCE antibody (350-400) (STJ502516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-PRKCE antibody (350-400) {Biotin} (STJ502517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-PRKCE antibody (350-400) {FITC} (STJ502518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Western blot analysis of various lysates, using PKC Epsilon Rabbit monoclonal antibody (STJ11105207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PRKCE antibody (488-737 aa) [S5207RM] (STJ11105207)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human stomach using PKC epsilon antibody (STJ25131) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCE antibody (488-737) (STJ25131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Mouse lung, using PKC epsilon Rabbit monoclonal antibody (STJ11102217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRKCE antibody (500-600) [S7MR] (STJ11102217)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Western blot analysis of 453 cells using PKC Epsilon Polyclonal Antibody diluted at 1：500

Anti-PRKCE antibody (688-737 aa) (STJ95128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of PKC gamma in rat brain lysates using PKC gamma antibody.

Anti-PRKCG antibody (484-683 aa) [R06-8C6] (STJA0011802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£264.50

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Western blot analysis of A549, THP-1, K562, mouse brain, mouse skeletal muscle, mouse testis cells using PKC Gamma Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PRKCG antibody (521-570 aa) (STJ96513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using PKC gamma Rabbit pAb (STJ110231) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.

Anti-PRKCG antibody (558-697) (STJ110231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PKC gamma Rabbit mAb (STJ11102494) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PRKCG antibody (604-697) [S4MR] (STJ11102494)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Anti-PRKCH antibody (1-50) (STJ502519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PRKCH antibody (1-50) {Biotin} (STJ502522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (1-50) {FITC} (STJ502523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (110-160) (STJ502520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PRKCH antibody (110-160) {Biotin} (STJ502524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (110-160) {FITC} (STJ502525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (500-550) (STJ502521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PRKCH antibody (500-550) {Biotin} (STJ502526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (500-550) {FITC} (STJ502527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKCH antibody (STJ191981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-PRKCI antibody (STJ191982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PRKCQ Antibody (STJ28397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRKCQ antibody (1-230) (STJ28397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 cells using PKC Theta Polyclonal Antibody

Anti-PRKCQ antibody (504-553 aa) (STJ95132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, treated with PMA 200nM 30', using PKC thet Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PRKCQ antibody (643-692 aa) (STJ95133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PRKCQ antibody (STJA0009845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using PKC zeta Rabbit monoclonal antibody (STJ11105217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : HELExposure time: 180s.

Anti-PRKCZ antibody (1-100 aa) [S5217RM] (STJ11105217)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using PKC zeta Rabbit polyclonal antibody (STJ116111) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PRKCZ antibody (476-547) (STJ116111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PKC zeta Rabbit polyclonal antibody (STJ28282) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKCZ antibody (476-547) (STJ28282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PKC Zeta Polyclonal Antibody diluted at 1：2000

Anti-PRKCZ antibody (526-575 aa) (STJ95130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of extracts of HepG2 cells, using PRKG1 antibody (STJ25143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-PRKG1 antibody (1-300) (STJ25143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-PRKG1 Antibody (STJ502528) with PKGA peptide. Antibody at 1:500 dilution in buffer.

Anti-PRKG1 antibody (609-659) (STJ502528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-PRKG1 antibody (609-659) {Biotin} (STJ502529)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKG1 antibody (609-659) {FITC} (STJ502530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-PRKG1 antibody (STJ191983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse lung using 3 Mu g PRKG2 antibody (STJ11100525). Western blot was performed from the immunoprecipitate using PRKG2 antibody (STJ11100525) at a dilution of 1:1000.

Anti-PRKG2 antibody (1-160) (STJ11100525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using cGKII Polyclonal Antibody

Anti-PRKG2 antibody (391-440 aa) (STJ92257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of A549 cells using cGKII Polyclonal Antibody diluted at 1：1000

Anti-PRKG2 antibody (92-141 aa) (STJ92256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PRKX Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PRKX antibody (251-300 aa) (STJ95224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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