• Confocal imaging of paraffin-embedded Mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of Human brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.
  • Immunohistochemistry analysis of Neurofilament L in paraffin-embedded mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Neurofilament L in paraffin-embedded rat brain using Neurofilament L Rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Neurofilament L in paraffin-embedded mouse brain using Neurofilament L Rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from SH-SY5Y cells using Neurofilament L Rabbit monoclonal antibody (STJ11103250) at 1:200000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:30s.

Anti-Neurofilament L antibody (60-250) [S0MR] (STJ11103250)

SKU:
STJ11103250

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Neurofilament L (60-250) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFL
Gene ID: 4747
Uniprot ID: NFL_HUMAN
Immunogen Region: 60-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2).
Immunogen Sequence: SSGSLMPSLENLDLSQVAAI SNDLKSIRTQEKAQLQDLND RFASFIERVHELEQQNKVLE AELLVLRQKHSEPSRFRALY EQEIRDLRLAAEDATNEKQA LQGEREGLEETLRNLQARYE EEVLSREDAEGRLMEARKGA DEAALARAELEKRIDSLMDE ISFLKKVHEEEIAELQAQIQ YAQISVEMDVT
Post Translational Modifications O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Neurofilament Light Polypeptide antibody
Anti-Nf-L antibody
Anti-68 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet L Protein antibody
Anti-NEFL antibody
Anti-NF68 antibody
Anti-NFL antibody

Information sourced from Uniprot.org

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