• Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3 (NRG3) ) antibody (STJ11103119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Neuregulin-3 (Neuregulin-3 (NRG3) ) antibody (STJ11103119) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Neuregulin-3 antibody (600-720) [S9MR] (STJ11103119)

SKU:
STJ11103119

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Neuregulin-3 (NRG3) (600-720) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRG3
Gene ID: 10718
Uniprot ID: NRG3_HUMAN
Immunogen Region: 600-720
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-720 of human Neuregulin-3 (Neuregulin-3 (NRG3) ) (P56975).
Immunogen Sequence: IIPSVGLEETCLQMPGISEV KSIKWCKNSYSADVVNVSIP VSDCLIAEQQEVKILLETVQ EQIRILTDARRSEDYELASV ETEDSASENTAFLPLSPTAK SEREAQFVLRNEIQRDSALT K
Tissue Specificity Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
Post Translational Modifications Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. Extensive glycosylation precedes the proteolytic cleavage. Isoform 3 is glycosylated.
Function Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
Protein Name Pro-Neuregulin-3 - Membrane-Bound Isoform
Pro-Nrg3 Cleaved Into - Neuregulin-3
Nrg-3
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1236394
Reactome: R-HSA-1250196
Reactome: R-HSA-1250342
Reactome: R-HSA-1250347
Reactome: R-HSA-1251985
Reactome: R-HSA-1257604
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8863795
Reactome: R-HSA-9664565
Reactome: R-HSA-9665686
Cellular Localisation Pro-Neuregulin-3
Membrane-Bound Isoform: Cell Membrane
Single-Pass Type I Membrane Protein
Does Not Seem To Be Active
Neuregulin-3: Secreted
Isoform 3: Cell Membrane
Isoform 3 Is Also Proteolytically Released As A Soluble Form
Alternative Antibody Names Anti-Pro-Neuregulin-3 - Membrane-Bound Isoform antibody
Anti-Pro-Nrg3 Cleaved Into - Neuregulin-3 antibody
Anti-Nrg-3 antibody
Anti-NRG3 antibody

Information sourced from Uniprot.org

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