Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using HCRTR2 antibody (STJ23927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HCRTR2 antibody (380-444) (STJ23927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-HDAC1 (PTR1326) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 55kDaObserved band size: 62kDa

Anti-HDAC1 antibody (300-482) [PTR1326] (STJA0006279)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300ug extracts of 293T cells using 0. 5ug HDAC1 antibody (STJ11101705). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ11101705) at a dilition of 1:1000.

Anti-HDAC1 antibody (350-450) [S5MR] (STJ11101705)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using HDAC1 Polyclonal Antibody diluted at 1：500

Anti-HDAC1 antibody (387-436 aa) (STJ93477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells, using 3 Mu g [KD Validated] HDAC1 Rabbit polyclonal antibody (STJ23928). Western blot was performed from the immunoprecipitate using HDAC1 antibody (STJ23928) at a dilution of 1:1000.

Anti-HDAC1 antibody (393-482) (STJ23928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Histone deacetylase 1 Polyclonal Antibody diluted at 1：1000

Anti-HDAC1 antibody (433-482 aa) (STJ93510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HDAC1 antibody (Internal Region) (STJ13101199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, HDAC1 Mouse monoclonal antibody (4E1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-HDAC1 antibody [4E1] (STJ97722)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from wild type (WT) and HDAC10 knockdown (KD) 293T (KD) cells, using HDAC10 Rabbit polyclonal antibody (STJ11105309) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HDAC10 antibody (1-100 aa) (STJ11105309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various cells using Histone deacetylase 10 Polyclonal Antibody diluted at 1：1000

Anti-HDAC10 antibody (10-59 aa) (STJ93511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-HDAC10 antibody (STJA0005751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Western blot analysis of lysates from Mouse brain using HDAC11 Rabbit mAb (STJ11107108) at 1:1000 dilution incubated at room temperature for 1. 5 hours. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.

Anti-HDAC11 antibody (1-347 aa) [SRM] (STJ11107108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HDAC11 antibody (170-220 aa) (STJ194849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HDAC11 antibody (STJ27893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HDAC11 antibody (270-347) (STJ27893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HDAC11 antibody [SAA1877] (STJA0020305)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC2 antibody (STJ11101736). Western blot was performed from the immunoprecipitate using HDAC2 antibody (STJ11101736) at a dilution of 1:1000.

Anti-HDAC2 antibody (1-100) [S6MR] (STJ11101736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using HDAC2 Polyclonal Antibody diluted at 1：2000

Anti-HDAC2 antibody (360-409 aa) (STJ93478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g HDAC2 antibody (STJ23929). Western blot was performed from the immunoprecipitate using HDAC2 antibody (STJ23929) at a dilution of 1:1000.

Anti-HDAC2 antibody (390-488) (STJ23929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from H460 cells, using HDAC2 Rabbit pAb (STJ111039) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HDAC2 antibody (429-488) (STJ111039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of mouse liver, using HDAC2 antibody (STJ115577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC2 antibody (429-488) (STJ115577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using HDAC3 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , C6 (3) and COS (4) cell lysate.

Anti-HDAC3 antibody (224-428 aa) [7G6C5] (STJ98129)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-HDAC3 antibody (299-428) (STJ23930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (STJ114416) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HDAC3 antibody (329-428) (STJ114416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HDAC3 Rabbit polyclonal antibody (STJ118890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC3 antibody (329-428) (STJ118890)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g HDAC3 Rabbit monoclonal antibody (STJ11101684). Western blot was performed from the immunoprecipitate using HDAC3 Rabbit monoclonal antibody (STJ11101684) at a dilution of 1:1000.

Anti-HDAC3 antibody (329-428) [S4MR] (STJ11101684)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using HDAC3 Polyclonal Antibody diluted at 1：1000

Anti-HDAC3 antibody (379-428 aa) (STJ93479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using SMAP45 Polyclonal Antibody diluted at 1：2000

Anti-HDAC3 antibody (379-428 aa) (STJ95704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of HDAC3 in (1) HeLa lysates; (2) NIH/3T3 lysates using HDAC3 antibody.

Anti-HDAC3 antibody (400-428 aa) [R07-8E9] (STJA0014653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis using HDAC3 monoclonal antibody against THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3, HeLa cell lysate.

Anti-HDAC3 antibody (STJ98496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Rabbit

£144.00 - £348.00

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Western blot detection of HDAC3 in THP-1, LNCAP, MCF7, PC12, 293, Jurkat, 3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa.

Anti-HDAC3 antibody [3G3-H6-H10] (STJ99157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of various lysates, using HDAC4 antibody (STJ11101423) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HDAC4 antibody (1-100) [S3MR] (STJ11101423)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of HDAC4 in paraffin-embedded mouse brain using HDAC4 Rabbit polyclonal antibody (STJ113153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC4 antibody (451-650) (STJ113153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using HDAC4 Rabbit polyclonal antibody (STJ29788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC4 antibody (530-630) (STJ29788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HDAC4 Polyclonal Antibody diluted at 1：2000

Anti-HDAC4 antibody (598-647 aa) (STJ93480)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using HDAC4 monoclonal antibody against Jurkat, K562 cell lysate.

Anti-HDAC4 antibody (N-Term) (STJ98497)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of HDAC4 in Jurkat, Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa.

Anti-HDAC4 antibody [4A3-H7-C6] (STJ99035)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using NY-CO-9 Polyclonal Antibody

Anti-HDAC5 antibody (1073-1122) (STJ94582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using HDAC5 antibody (STJ29269). Blue: DAPI for nuclear staining.

Anti-HDAC5 antibody (350-450) (STJ29269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HDAC5 Polyclonal Antibody

Anti-HDAC5 antibody (464-513 aa) (STJ93481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A-549 cells using HDAC5 Rabbit polyclonal antibody (STJ113548).

Anti-HDAC5 antibody (500-600) (STJ113548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC6 antibody (STJ11103524). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ11103524) at a dilution of 1:1000.

Anti-HDAC6 antibody (1-100) [S4MR] (STJ11103524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using HDAC6 antibody (STJ23932) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HDAC6 antibody (1-200) (STJ23932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HDAC6 (ABT-HDAC6) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 131kDaObserved band size: 130, 180kDa

Anti-HDAC6 antibody (1100-1215 aa) [ABT-HDAC6] (STJ196790)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse

£174.00 - £252.00

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Western blot analysis of various cells using Histone deacetylase 6 Polyclonal Antibody diluted at 1：2000

Anti-HDAC6 antibody (1166-1215 aa) (STJ93512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using HDAC6 antibody (STJ113792).

Anti-HDAC6 antibody (160-470) (STJ113792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts from K-562 cells, using HDAC6 Rabbit monoclonal antibody (STJ11104740) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-HDAC6 antibody (600-700) [S4740RM] (STJ11104740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of Hela cell. 1, HDAC6 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HDAC6 antibody (7-56 aa) (STJ93483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g HDAC6 antibody (STJ113047). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ113047) at a dilution of 1:1000.

Anti-HDAC6 antibody (836-1104) (STJ113047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options