• Western blot analysis of extracts of various cell lines, using FGF1 antibody (STJ23651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using FGF1 antibody (STJ23651) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using FGF1 antibody (STJ23651) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HepG2 cells using FGF1 rabbit polyclonal antibody (STJ23651) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-FGF1 antibody (16-155) (STJ23651)

SKU:
STJ23651

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FGF1 (16-155) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGF1
Gene ID: 2246
Uniprot ID: FGF1_HUMAN
Immunogen Region: 16-155
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 16-155 of human FGF1 (NP_000791.1).
Immunogen Sequence: FNLPPGNYKKPKLLYCSNGG HFLRILPDGTVDGTRDRSDQ HIQLQLSAESVGEVYIKSTE TGQYLAMDTDGLLYGSQTPN EECLFLERLEENHYNTYISK KHAEKNWFVGLKKNGSCKRG PRTHYGQKAILFLPLPVSSD
Tissue Specificity Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
Post Translational Modifications In the nucleus, phosphorylated by PKC/PRKCD.
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.
Protein Name Fibroblast Growth Factor 1
Fgf-1
Acidic Fibroblast Growth Factor
Afgf
Endothelial Cell Growth Factor
Ecgf
Heparin-Binding Growth Factor 1
Hbgf-1
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839122
Reactome: R-HSA-1839130
Reactome: R-HSA-190322
Reactome: R-HSA-190370
Reactome: R-HSA-190371
Reactome: R-HSA-190372
Reactome: R-HSA-190373
Reactome: R-HSA-190375
Reactome: R-HSA-190377
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5654687
Reactome: R-HSA-5654688
Reactome: R-HSA-5654689
Reactome: R-HSA-5654693
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655253
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Reactome: R-HSA-8851708
Cellular Localisation Secreted
Cytoplasm
Cell Cortex
Cytosol
Nucleus
Lacks A Cleavable Signal Sequence
Within The Cytoplasm
It Is Transported To The Cell Membrane And Then Secreted By A Non-Classical Pathway That Requires Cu(2+) Ions And S100a13
Secreted In A Complex With Syt1
Binding Of Exogenous Fgf1 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf1 Across Endosomal Membrane Into The Cytosol
Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery
Involving Proteins Kpna1 And Kpnb1
As Well As Lrrc59
Alternative Antibody Names Anti-Fibroblast Growth Factor 1 antibody
Anti-Fgf-1 antibody
Anti-Acidic Fibroblast Growth Factor antibody
Anti-Afgf antibody
Anti-Endothelial Cell Growth Factor antibody
Anti-Ecgf antibody
Anti-Heparin-Binding Growth Factor 1 antibody
Anti-Hbgf-1 antibody
Anti-FGF1 antibody
Anti-FGFA antibody

Information sourced from Uniprot.org

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