Programmed Cell Death | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using PSME1 antibody (STJ27311) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-PSME1 antibody (1-249) (STJ27311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PSME1 antibody (60-110 aa) (STJ193989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from wild type (WT) and PSME2 knockout (KO) 293T cells, using [KO Validated] PSME2 Rabbit polyclonal antibody (STJ11100740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ11100740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of extracts from wild type (WT) and PSME2 knockout (KO) 293T (KO) cells, using PSME2 antibody (STJ115590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ115590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of extracts from wild type (WT) and PSME2 knockout (KO) 293T (KO) cells, using PSME2 antibody (STJ27508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PSME2 antibody (1-239) (STJ27508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Anti-PSME2 antibody (STJA0008916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of PSME3 in paraffin-embedded rat kidney using PSME3 Rabbit polyclonal antibody (STJ114570) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PSME3 antibody (200-254) (STJ114570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PSME3 antibody (200-254) (STJ119994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis using PA28 Gamma monoclonal antibody against HepG2, Jurkat, K562 cell lysate.

Anti-PSME3 antibody (STJ98526)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Dog/Pig

$194.40 - $469.80

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Immunohistochemistry analysis of PSME4 in paraffin-embedded Human esophageal using PSME4 Rabbit polyclonal antibody (STJ115757) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PSME4 antibody (1634-1843) (STJ115757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-PSME4 antibody (STJA0007804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates, using PSMF1 rabbit polyclonal antibody (STJ27500) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMF1 antibody (1-271) (STJ27500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-PSMF1 antibody (STJA0008039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-PTK2 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PTK2 antibody (690aa C-Term) (STJ140154)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$182.93 - $532.58

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Confocal imaging of HeLa cells using FAK Rabbit monoclonal antibody (STJ112977, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-PTK2 antibody (700-800) [S7MR] (STJ112977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit polyclonal antibody (STJ113175) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ113175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Mouse

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using FAK Rabbit polyclonal antibody (STJ114921) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTK2 antibody (734-834) (STJ114921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of rat tissue with Anti-PTK2 primary antibody (STJ500981) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-PTK2 antibody (740-790) (STJ500981)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-PTK2 antibody (740-790) {Biotin} (STJ500982)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-PTK2 antibody (740-790) {FITC} (STJ500983)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of Hela cell. 1, FAK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PTK2 antibody (809-858 aa) (STJ93024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis using FAK monoclonal antibody against A549 (1) and NIH/3T3 (2) cell lysate.

Anti-PTK2 antibody [10H7] (STJ98061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$194.40 - $469.80

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Western blot analysis using FAK monoclonal antibody against FAK-hIgGFc transfected HEK293 cell lysate.

Anti-PTK2 antibody [10H7A6] (STJ98062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Anti-PTK2 antibody [10H7E9] (STJ98063)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot detection of FAK in COS7, HUVEC, A549 and Hela cell lysates using FAK mouse mAb (1:1000 diluted).Predicted band size: 125KDa.Observed band size:125KDa.

Anti-PTK2 antibody [3D8-H11-E12] (STJ99191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis using FAK monoclonal antibody against truncated FAK recombinant protein.

Anti-PTK2 antibody [4A9D6] (STJ98060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Immunohistochemistry analysis of Bax. Sample: Human colon cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:200, RT, 1h.

Anti-Recombinant-Bax antibody [SR0671] (STJA0010671)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-Bcl-2 antibody [SR0674] (STJA0010674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

$230.18 - $315.23

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Anti-Recombinant-beta Catenin antibody [SR0538] (STJA0010538)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-beta Catenin antibody [SR0543] (STJA0010543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-Caspase-1 antibody [SR0666] (STJA0010666)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-Caspase-7 antibody [SM0683] (STJA0010683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Anti-Recombinant-Granzyme B antibody [SM0562] (STJA0010562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Anti-Recombinant-Granzyme B antibody [SM0563] (STJA0010563)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

$230.18 - $315.23

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Sample: Human breast cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Recombinant-p40-DeltaNp63 antibody [SM0630] (STJA0010630)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Western blot analysis of p40-DeltaNp63. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG, 1:3000, RT, 1h

Anti-Recombinant-p40-DeltaNp63 antibody [SR0525] (STJA0010525)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human

$230.18 - $315.23

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Immunohistochemistry analysis of p63. Sample: Human cervical carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-mouse lgG, Ready to use, RT, 20min.

Anti-Recombinant-p63 antibody [SM0534] (STJA0010534)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-p63 antibody [SM0668] (STJA0010668)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-p63 antibody [SR0711] (STJA0010711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Mouse/Rat

$230.18 - $315.23

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Immunohistochemistry analysis of Granzyme B. Sample: Rat thymus, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:800, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.

Anti-Recombinant-Phospho-Granzyme B antibody [SR0537] (STJA0010537)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Rat

$230.18 - $315.23

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Anti-Recombinant-Phospho-STAT3-Y705 antibody [SR0523] (STJA0010523)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Anti-Recombinant-ZO1 tight junction protein antibody [SR0620] (STJA0010620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Confocal imaging of L-929 cells using RIP3 Rabbit mAb (STJ11107387, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Î±-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-RIP3 antibody (1-287 aa) [SRM] (STJ11107387)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

$174.83 - $775.58

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Immunofluorescence analysis of NIH/3T3 cells using RIPK1/RIP Rabbit polyclonal antibody (STJ29550) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RIPK1 antibody (100-200) (STJ29550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g RIPK1/RIP Rabbit monoclonal antibody (STJ11101710). Western blot was performed from the immunoprecipitate using RIPK1/RIP Rabbit monoclonal antibody (STJ11101710) at a dilution of 1:500.

Anti-RIPK1 antibody (289-490) [S0MR] (STJ11101710)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$197.78 - $867.38

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Anti-RIPK1 antibody (620-670) (STJ502798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$577.13

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Anti-RIPK1 antibody (620-670) {Biotin} (STJ502799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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Anti-RIPK1 antibody (620-670) {FITC} (STJ502800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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