Metabolism | Research Area | St John's Laboratory

Anti-PCBD1 antibody [SRM13668] (STJ11108398)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using PCCA Rabbit pAb (STJ111719) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-PCCA antibody (449-728) (STJ111719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Anti-PCCA antibody (STJA0007001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of extracts of various cell lines, using PCCB antibody (STJ27368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PCCB antibody (260-539) (STJ27368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€132.60 - €606.60

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Anti-PCCB antibody (STJA0008383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-PCCB antibody [SRM14784] (STJ11109514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of extracts of various cell lines, using PCK1 antibody (STJ24916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PCK1 antibody (490-622) (STJ24916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of HeLa, MCF7 cells using PEPCK-C Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PCK1 antibody (491-540 aa) (STJ96601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of HepG2, using PCK1 antibody (STJ11104742) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-PCK1 antibody (523-622) [S4742RM] (STJ11104742)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Anti-PCK1 antibody [R01-4Q5] (STJA0036757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

€160.20 - €337.80

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Anti-PCK1 antibody [R09-1T-3] (STJA0033099)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat

€257.40 - €370.20

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Western blot analysis of lysates from Hep G2 cells using PEPCK/PCK2 Rabbit pAb (STJ110744) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PCK2 antibody (1-280) (STJ110744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 antibody (STJ11104393). Western blot was performed from the immunoprecipitate using PEPCK/PEPCK/PCK2 antibody (STJ11104393) at a dilution of 1:1000.

Anti-PCK2 antibody (1-280) (STJ11104393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis using PEPCK monoclonal antibody against ZR75-1, HepG2, HeLa cell lysate.

Anti-PCK2 antibody (C-Term) (STJ98530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

€178.80 - €432.00

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Anti-PCK2 antibody [3F7] (STJ16103462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat/Monkey

€895.80

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Anti-PCK2 antibody [R07-8K6] (STJA0034693)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-PCTP antibody (STJA0009152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using PCYT1A Rabbit polyclonal antibody (STJ11102806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCYT1A antibody (1-100) (STJ11102806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€132.60 - €606.60

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Western blot analysis of extracts of K-562 cells, using PCYT1A Rabbit monoclonal antibody (STJ11103592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PCYT1A antibody (268-367) [S2MR] (STJ11103592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PCYT1A antibody (STJ193012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-PCYT1B antibody (89-139 aa) (STJ198077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using PCYT2 Rabbit polyclonal antibody (STJ117504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PCYT2 antibody (1-389) (STJ117504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Anti-PCYT2 antibody (STJA0007833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of extracts of various cell lines, using PDHA1 antibody (STJ11104699) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PDHA1 antibody (248-348) [S4699RM] (STJ11104699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Immunohistochemistry analysis of PDHA1 in paraffin-embedded human thyroid cancer using PDHA1 Rabbit polyclonal antibody (STJ24939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PDHA1 antibody (30-390) (STJ24939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000

Anti-PDHA1 antibody (314-363 aa) (STJ95006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Confocal imaging of C2C12 cells using PDHA1 Rabbit monoclonal antibody (STJ11101429, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDHA1 antibody (50-150) [S9MR] (STJ11101429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis using PDH-E1 Alpha monoclonal antibody against A549, HeLa cell lysate.

Anti-PDHA1 antibody (C-Term) (STJ98528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

€178.80 - €432.00

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Anti-PDHA1 antibody (STJA0010259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.

Anti-PDHA1 antibody [3H2-F8-B5] (STJ99056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse

€178.80 - €432.00

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Western blot analysis of various lysates using PDHA2 Rabbit pAb (STJ111984) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-PDHA2 antibody (119-388) (STJ111984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHA2 antibody (310-390 aa) (STJ191234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of extracts of various cell lines, using PDHB antibody (STJ29023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (1-300) (STJ29023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHB antibody (160-240 aa) (STJ191235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of PDHB in paraffin-embedded rat kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-PDHB antibody (260-359) [S3MR] (STJ11102123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g PDHX antibody (STJ28509). Western blot was performed from the immunoprecipitate using PDHX antibody (STJ28509) at a dilution of 1:1000.

Anti-PDHX antibody (1-300) (STJ28509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHX antibody (24-74 aa) (STJ195078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-PDHX antibody (54-501aa) [AT1E11] (STJA0041867)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

€263.40 - €399.00

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Western blot analysis of various lysates using PDK1/PDHK1 Rabbit monoclonal antibody (STJ11105443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PDK1 antibody (1-100) [S5443RM] (STJ11105443)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK1 antibody (337-436) (STJ24945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis of various lysates, using PDK1/PDHK1 antibody (STJ11104825) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDK1 antibody (337-436) [S4825RM] (STJ11104825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €665.40

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Western blot analysis of various lysates, using PDK1/PDHK1 Rabbit mAb (STJ11102396) at 1:20000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PDK1 antibody (337-436) [S6MR] (STJ11102396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of various cells using PDK1 Polyclonal Antibody

Anti-PDK1 antibody (80-160 aa) (STJ95009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-PDK1 antibody (STJA0038032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

€225.00 - €337.80

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Western blot analysis using PDK1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , Jurkat (3) , HepG2 (4) , PC-12 (5) , and Cos7 (6) cell lysate.

Anti-PDK1 antibody [4A11] (STJ98315)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

€178.80 - €432.00

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Anti-PDK1 antibody [PT1266R] (STJA0040161)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-PDK1 antibody [R02-9K3] (STJA0036472)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-PDK1 antibody [SAA1680] (STJA0020108)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€238.20 - €1,404.60

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