Anti-PCCA antibody (449-728) (STJ111719)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PCCA (449-728) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:3000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCCA |
| Gene ID | 5095 |
| Uniprot ID | PCCA_HUMAN |
| Immunogen Region | 449-728 |
| Immunogen Sequence | ISKLITYGSDRTEALKRMAD ALDNYVIRGVTHNIALLREV IINSRFVKGDISTKFLSDVY PDGFKGHMLTKSEKNQLLAI ASSLFVAFQLRAQHFQENSR MPVIKPDIANWELSVKLHDK VHTVVASNNGSVFSVEVDGS KLNVTSTWNLASPLLSVSVD GTQRTVQCLSREAGGNMSIQ FLGTVYKVNILTRLAAELNK FMLEKVTEDTSSVLRSPMPG VVVAVSVKPGDAVAEGQEI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 449-728 of human PCCA (NP_000273.2). |
Additional Info
| Post Translational Modifications | Acetylated. The biotin cofactor is covalently attached to the C-terminal biotinyl-binding domain and is required for the catalytic activity. Biotinylation is catalyzed by HLCS. |
| Function | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA. |
| Protein Name | Propionyl-Coa Carboxylase Alpha Chain - MitochondrialPccase Subunit AlphaPropanoyl-Coa -Carbon Dioxide Ligase Subunit Alpha |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-71032 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Propionyl-Coa Carboxylase Alpha Chain - Mitochondrial antibodyAnti-Pccase Subunit Alpha antibodyAnti-Propanoyl-Coa -Carbon Dioxide Ligase Subunit Alpha antibodyAnti-PCCA antibody |
Information sourced from Uniprot.org