• Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 antibody (STJ11104393). Western blot was performed from the immunoprecipitate using PEPCK/PEPCK/PCK2 antibody (STJ11104393) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using PEPCK/PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded mouse kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PEPCK/PCK2 in paraffin-embedded rat kidney using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and PEPCK/PCK2 Rabbit polyclonal antibody knockout (KO) 293T cells, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates, using [KO Validated] PEPCK/PCK2 Rabbit polyclonal antibody (STJ11104393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PCK2 antibody (1-280) (STJ11104393)

SKU:
STJ11104393

£134.50 - £666.50
Current Stock:
Host: Rabbit
Applications: WB/IF/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phosphoenolpyruvate Carboxykinase Gtp-Mitochondrial (1-280) is suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IHC-P 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCK2
Gene ID: 5106
Uniprot ID: PCKGM_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PCK2 (NP_004554.2).
Immunogen Sequence: MAALYRPGLRLNWHGLSPLG WPSCRSIQTLRVLSGDLGQL PTGIRDFVEHSARLCQPEGI HICDGTEAENTATLTLLEQQ GLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTV QLPPGGARGQLGNWMSPADF QRAVDERFPGCMQGRTMYVL PFSMGPVGSPLSRIGVQLTD SAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTG QGEPVSQWPCNPEKTLIGH
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Protein Name Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial
Pepck-M
Database Links Reactome: R-HSA-70263
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibody
Anti-Pepck-M antibody
Anti-PCK2 antibody
Anti-PEPCK2 antibody

Information sourced from Uniprot.org

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