Anti-PCCB antibody (260-539) (STJ27368)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PCCB (260-539) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCCB |
| Gene ID | 5096 |
| Uniprot ID | PCCB_HUMAN |
| Immunogen Region | 260-539 |
| Immunogen Sequence | AFENDVDALCNLRDFFNYLP LSSQDPAPVRECHDPSDRLV PELDTIVPLESTKAYNMVDI IHSVVDEREFFEIMPNYAKN IIVGFARMNGRTVGIVGNQP KVASGCLDINSSVKGARFVR FCDAFNIPLITFVDVPGFLP GTAQEYGGIIRHGAKLLYAF AEATVPKVTVITRKAYGGAY DVMSSKHLCGDTNYAWPTAE IAVMGAKGAVEIIFKGHENV EAAQAEYIEKFANPFPAAV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 260-539 of human PCCB (NP_000523.2). |
Additional Info
| Function | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA. |
| Protein Name | Propionyl-Coa Carboxylase Beta Chain - MitochondrialPccase Subunit BetaPropanoyl-Coa -Carbon Dioxide Ligase Subunit Beta |
| Database Links | Reactome: R-HSA-196780Reactome: R-HSA-3371599Reactome: R-HSA-71032Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Propionyl-Coa Carboxylase Beta Chain - Mitochondrial antibodyAnti-Pccase Subunit Beta antibodyAnti-Propanoyl-Coa -Carbon Dioxide Ligase Subunit Beta antibodyAnti-PCCB antibody |
Information sourced from Uniprot.org