Metabolism Of Proteins | Research Area | St John's Laboratory

Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells using 3 Mu g PDHA1 antibody (STJ11104401). Western blot was performed from the immunoprecipitate using PDHA1 antibody (STJ11104401) at a dilution of 1:1000.

Anti-PDHA1 antibody (30-390) (STJ11104401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using PDHA1 Rabbit polyclonal antibody (STJ119549) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDHA1 antibody (30-390) (STJ119549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of PDHA1 in paraffin-embedded human thyroid cancer using PDHA1 Rabbit polyclonal antibody (STJ24939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PDHA1 antibody (30-390) (STJ24939)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000

Anti-PDHA1 antibody (314-363 aa) (STJ95006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of C2C12 cells using PDHA1 Rabbit monoclonal antibody (STJ11101429, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PDHA1 antibody (50-150) [S9MR] (STJ11101429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis using PDH-E1 Alpha monoclonal antibody against A549, HeLa cell lysate.

Anti-PDHA1 antibody (C-Term) (STJ98528)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Pig

£144.00 - £348.00

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Anti-PDHA1 antibody (STJA0010259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa.

Anti-PDHA1 antibody [3H2-F8-B5] (STJ99056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using PDHB antibody (STJ29023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDHB antibody (1-300) (STJ29023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PDHB antibody (160-240 aa) (STJ191235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of PDHB in paraffin-embedded rat kidney tissue using PDHB Rabbit monoclonal antibody (STJ11102123) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-PDHB antibody (260-359) [S3MR] (STJ11102123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human placenta using ERp57 Rabbit monoclonal antibody (STJ11102061) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA3 antibody (100-200) [S1MR] (STJ11102061)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of A549, HT29, K562 cells using ERp57 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PDIA3 antibody (111-160 aa) (STJ96585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using ERp57 antibody (STJ24942) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA3 antibody (206-505) (STJ24942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Endogenous ERP57-endoplasmic reticulum lumen marker detected at 1:500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDIA3 antibody (300aa C-Term) (STJ140035)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Anti-PDIA3 antibody (300aa C-Term) (STJ140036)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Confocal imaging of HeLa cells using PDIA6 Rabbit monoclonal antibody (STJ11102028, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-PDIA6 antibody (1-100) [S8MR] (STJ11102028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from wild type (WT) and PDIA6 knockout (KO) 293T cells, using [KO Validated] PDIA6 Rabbit polyclonal antibody (STJ11100065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PDIA6 antibody (20-300) (STJ11100065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using PDIA6 Antibody (STJ29135) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PDIA6 antibody (20-300) (STJ29135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PDIA6 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PDIA6 antibody (220aa C-Term) (STJ140160)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-PDIA6 antibody (STJA0006902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using PDK1/PDHK1 Rabbit monoclonal antibody (STJ11105443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PDK1 antibody (1-100) [S5443RM] (STJ11105443)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of PC-12 cells using PDK1/PDHK1 Rabbit polyclonal antibody (STJ24945) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDK1 antibody (337-436) (STJ24945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PDK1/PDHK1 antibody (STJ11104825) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDK1 antibody (337-436) [S4825RM] (STJ11104825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using PDK1/PDHK1 rabbit monoclonal antibody (STJ11102396) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PDK1 antibody (337-436) [S6MR] (STJ11102396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using PDK1 Polyclonal Antibody

Anti-PDK1 antibody (80-160 aa) (STJ95009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using PDK1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , Jurkat (3) , HepG2 (4) , PC-12 (5) , and Cos7 (6) cell lysate.

Anti-PDK1 antibody [4A11] (STJ98315)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of paraffin-embedded Rat heart tissue using PENK Rabbit polyclonal antibody (STJ11105654) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PENK antibody (150-267) (STJ11105654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PENK antibody (STJ28224) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 20s.

Anti-PENK antibody (25-267) (STJ28224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of NT28 cells using Antibody diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-PENK antibody (51-100 aa) (STJ98660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Pepsinogen I antibody (1-388 aa) [SRM] (STJ11107294)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of U-87MG, using PEX10 antibody (STJ119243) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PEX10 antibody (10-160) (STJ119243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PEX10 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PEX10 antibody (183-232 aa) (STJ95035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PEX12 antibody (180-260 aa) (STJ191143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PEX12 Rabbit polyclonal antibody (STJ118502) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PEX12 antibody (290-359) (STJ118502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-PEX13 antibody (290-370 aa) (STJ191137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Peroxin 14 Polyclonal Antibody

Anti-PEX14 antibody (117-166 aa) (STJ95038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of mouse kidney, using PEX14 rabbit polyclonal antibody (STJ29475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PEX14 antibody (118-377) (STJ29475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of mouse-liver lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-PEX14 antibody (STJ99650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of PEX2 in paraffin-embedded human placenta using PEX2 Rabbit polyclonal antibody (STJ117503) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PEX2 antibody (1-130) (STJ117503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Peroxin 2 Polyclonal Antibody

Anti-PEX2 antibody (1-50 aa) (STJ95040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PFDN1 antibody (STJ113579) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PFDN1 antibody (1-122) (STJ113579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from RAW264.7 cells, using PFDN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-PFDN1 antibody (1-50 aa) (STJ95219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using PFDN2 Rabbit polyclonal antibody (STJ114159) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PFDN2 antibody (1-154) (STJ114159)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PFDN2 antibody (81-131 aa) (STJ194667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using PFDN4 Rabbit polyclonal antibody (STJ117495) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PFDN4 antibody (1-134) (STJ117495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PFDN4 antibody (65-115 aa) (STJ195434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of L929 cells using PFDN5 Rabbit polyclonal antibody (STJ116227) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PFDN5 antibody (1-154) (STJ116227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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