Immune System | Research Area | St John's Laboratory

Western blot analysis using Akt1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) and Jurkat (4) cell lysate.

Anti-AKT1 antibody [3A3] (STJ97820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Anti-AKT1 nanobody [SAA1080] (STJN000196)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-AKT1 nanobody [SAA1231] (STJN000197)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£378.50 - £1,632.50

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Immunofluorescence analysis of U2OS cells using Akt2 Rabbit polyclonal antibody (STJ118023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT2 antibody (100-200) (STJ118023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 293T cells, using AKT2 Rabbit polyclonal antibody (STJ119992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AKT2 antibody (381-481) (STJ119992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using AKT2 antibody (STJ22573) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AKT2 antibody (400-500) (STJ22573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

£144.00 - £348.00

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Western blot analysis of various lysates using AKT3 Rabbit polyclonal antibody (STJ114775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-AKT3 antibody (90-465) (STJ114775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using AKT3 antibody (STJ11104934) at 1:60000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AKT3 antibody (93-154 aa) [S4934RM] (STJ11104934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) Mouse Brain, 4) Rat Brain tissue, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-AKT3 antibody (STJ197445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein (1) and human ovary carcinoma tissue lysate (2).

Anti-AKT3 antibody [3B12F3F10/2D2B11B11/2D2B11H5] (STJ97822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-AKT3 nanobody [SAA1232] (STJN000317)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of various lysates using ALAD Rabbit pAb (STJ110696) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ALAD antibody (1-330) (STJ110696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALAD antibody (1-330) (STJ114271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using ALDH3B1 Rabbit pAb (STJ112450) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-ALDH3B1 antibody (1-80) (STJ112450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using ALDH3B1 Polyclonal Antibody diluted at 1：500

Anti-ALDH3B1 antibody (51-100 aa) (STJ91555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells using ALDOA antibody (STJ22584). Blue: DAPI for nuclear staining.

Anti-ALDOA antibody (1-348) (STJ22584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Aldolase A Polyclonal Antibody diluted at 1：1000

Anti-ALDOA antibody (1-50 aa) (STJ91556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using ALDOC Rabbit polyclonal antibody (STJ113223) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALDOC antibody (1-170) (STJ113223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of mouse liver, mouse spinal cord cells using Aldolase C Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ALDOC antibody (21-70 aa) (STJ96549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using ALOX15 Rabbit polyclonal antibody (STJ28944) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15 antibody (1-230) (STJ28944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15 antibody (156-206 aa) (STJ195947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of C6 cells using ALOX5 antibody (STJ22592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALOX5 antibody (1-200) (STJ22592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of U-2 OS cells using ALOX5 Rabbit monoclonal antibody (STJ11101834, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-ALOX5 antibody (100-200) [S4MR] (STJ11101834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Arachidonate 5 Lipoxygenase Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALOX5 antibody (246-295 aa) (STJ91392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Alpha-synuclein Mouse mAb (STJ11107379) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-Alpha-synuclein antibody (1-140 aa) [SMM] (STJ11107379)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Alpha-Synuclein antibody (1-140 aa) [SRM] (STJ11107343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-Alpha-Synuclein antibody (59-140 aa) [SRM] (STJ11106873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Alpha-Synuclein antibody [KM51] (STJ16101679)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£843.50

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Western blot analysis of various lysates using Alpha-Tubulin Rabbit pAb (STJ11103685) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£160.50 - £300.50

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Western blot analysis of extracts of various cell lines, using Alpha-Tubulin antibody (STJ11103692) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Alpha-Tubulin antibody (1-180) (STJ11103692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£160.50 - £300.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103689) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£96.50 - £392.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (STJ11103690) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) (STJ11103690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£207.50 - £392.50

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Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit mAb (STJ11103694) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Alpha-tubulin antibody (1-80) [S4MR] (STJ11103694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £662.50

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Western blot analysis of various lysates using Alpha-Tubulin Mouse mAb (STJ11107556) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Alpha-Tubulin antibody [SMM] (STJ11107556)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£64.50 - £300.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ALPK1 using ALPK1 Rabbit pAb (STJ11106978) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s. <br><b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>

Anti-ALPK1 antibody (1-473 aa) (STJ11106978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using Alpha-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ALPK1 antibody (11-60 aa) (STJ96366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ALPK1 Rabbit polyclonal antibody (STJ11103266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALPK1 antibody (340-500) (STJ11103266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-AMPD3 antibody (1-260) (STJ115425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates, using AMPD3 Rabbit polyclonal antibody (STJ28437) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AMPD3 antibody (1-260) (STJ28437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-AMPD3 antibody (280-360 aa) (STJ91578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Amyloid beta 40 antibody [SM4928] (STJA0004928)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-Amyloid Precursor Protein antibody (700-770) (STJ506314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-Amyloid Precursor Protein antibody (700-770) {Biotin} (STJ506315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Amyloid Precursor Protein antibody (700-770) {FITC} (STJ506316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates using ANAPC1 Rabbit pAb (STJ22606) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-ANAPC1 antibody (1-250) (STJ22606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APC1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ANAPC1 antibody (321-370 aa) (STJ91626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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