Immune System | Research Area | St John's Laboratory

Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0. 1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Rabbit antibody to LL-37 (STJ13100071) , diluted 50 ug/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclar cells.

Anti-LL-37 antibody (STJ13100071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-LL-37 antibody (STJ13100505)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Western blot analysis of MCF7, SW480, PC-3, SKOV3, mouse spleen, mouse brain, mouse liver, mouse thymus cells using Lamin B1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-LMNB1 antibody (391-440 aa) (STJ96545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (STJ24413) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LMNB1 antibody (397-588) (STJ24413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry of mouse spleen with Anti-LMNB1 primary antibody (STJ501624) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-LMNB1 antibody (450-500) (STJ501624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-LMNB1 antibody (450-500) {Biotin} (STJ501625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-LMNB1 antibody (450-500) {FITC} (STJ501626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-LMNB1 antibody (503-588) (STJ119228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Lamin B1 antibody western blot K562 rat brain C6 3T3 HeLa STJA0010488

Anti-LMNB1 antibody (527-540 aa) [R05-8D6] (STJA0010488)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£168.50 - £727.50

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Western blot detection of Lamin B1 in COS7, Molt-4, A549 and 3T3 cell lysates using Lamin B1 mouse mAb (dilution 1:500).Predicted band size:68kDa.Observed band size:68kDa.

Anti-LMNB1 antibody [5G8-D3-H7] (STJ99312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-LMNB1 antibody [7C11] (STJ96938)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 350} (STJA0006109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 405} (STJA0006110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 488} (STJA0006111)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 555} (STJA0006112)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 594} (STJA0006113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 647} (STJA0006114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 680} (STJA0006115)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {Biotin} (STJA0006116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {Cy3} (STJA0006117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {Cy5} (STJA0006118)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {FITC} (STJA0006119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMNB1 antibody [7C11] {HRP} (STJA0006120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£149.00 - £248.00

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Anti-LMO7 antibody (1310-1390 aa) (STJ190239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of LNPEP in paraffin-embedded human placenta using LNPEP Rabbit polyclonal antibody (STJ113875) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-LNPEP antibody (1-110) (STJ113875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-LNPEP antibody (705-755 aa) (STJ192906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using LNX1 rabbit polyclonal antibody (STJ28564) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LNX1 antibody (501-728) (STJ28564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-LNX1 antibody (527-577) (STJA0006514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LONRF1 antibody (690-740 aa) (STJ193810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-LOX-1 antibody [15C4] (STJA0027803)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human astrovirus-8

£166.50

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Anti-LOX-1 antibody [15C4] {APC} (STJA0029489)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human astrovirus-8

£228.50

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Anti-LOX-1 antibody [15C4] {FITC} (STJA0028523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human astrovirus-8

£201.50

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Anti-LOX-1 antibody [15C4] {PerCP} (STJA0030455)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human astrovirus-8

£215.50

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Anti-LOX-1 antibody [15C4] {PE} (STJA0031421)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human astrovirus-8

£228.50

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Anti-LOX1 antibody [R01-3G-1] (STJA0033749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£214.50 - £308.50

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Western blot analysis of various lysates, using LPCAT1 antibody (STJ27005) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LPCAT1 antibody (325-534) (STJ27005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-LPCAT1 antibody (STJA0006773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-LPCAT1 antibody (STJA0037977)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Anti-LPCAT1 antibody [R07-4K3] (STJA0034533)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£140.50 - £308.50

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Anti-LPO antibody (STJA0007758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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LRG1 antibody western blot human serum lysates STJA0011560

Anti-LRG1 antibody (282-293 aa) [R06-4I3] (STJA0011560)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from Human plasma, using LRG1 Rabbit pAb (STJ110160) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-LRG1 antibody (36-347) (STJ110160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using LRG1 polyclonal antibody STJ93953

Anti-LRG1 antibody (C-Term) (STJ93953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-LRG1 antibody [SAA1550] (STJA0019978)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-LRP/MVP antibody [LMR5] (STJ16100893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/FC

Reactivity :

Human

£525.50

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Anti-LRP/MVP antibody [MVP-37] (STJ16100902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/WB/FC

Reactivity :

Human

£525.50

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Anti-LRP/MVP antibody [SRM13430] (STJ11108160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-LRR1 antibody (116-166 aa) (STJ194025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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