• Western blot analysis of extracts of Human plasma, using LRG1 antibody (STJ110160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of paraffin-embedded mouse liver using LRG1 Rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver cells using LRG1 rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human liver using LRG1 Rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human liver cells using LRG1 rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat liver using LRG1 Rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver cells using LRG1 rabbit polyclonal antibody (STJ110160) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Human plasma, using LRG1 Rabbit polyclonal antibody (STJ110160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LRG1 antibody (36-347) (STJ110160)

SKU:
STJ110160

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Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LRG1 (36-347) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRG1
Gene ID: 116844
Uniprot ID: A2GL_HUMAN
Immunogen Region: 36-347
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-347 of human LRG1 (NP_443204.1).
Immunogen Sequence: VTLSPKDCQVFRSDHGSSIS CQPPAEIPGYLPADTVHLAV EFFNLTHLPANLLQGASKLQ ELHLSSNGLESLSPEFLRPV PQLRVLDLTRNALTGLPPGL FQASATLDTLVLKENQLEVL EVSWLHGLKALGHLDLSGNR LRKLPPGLLANFTLLRTLDL GENQLETLPPDLLRGPLQLE RLHLEGNKLQVLGKDLLLPQ PDLRYLFLNGNKLARVAAGA FQGLRQLDMLDLSNNSLAS
Tissue Specificity Plasma.
Protein Name Leucine-Rich Alpha-2-Glycoprotein
Lrg
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative Antibody Names Anti-Leucine-Rich Alpha-2-Glycoprotein antibody
Anti-Lrg antibody
Anti-LRG1 antibody
Anti-LRG antibody

Information sourced from Uniprot.org

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