Immune System | Research Area | St John's Laboratory

Western blot analysis of lysates from A-431 cells, using Phospho-SHC1-Y427 Rabbit pAb (STJ11102671) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SHC1-Y427 antibody (STJ11102671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from 293 cells using Phospho-SHC1-Y427 Rabbit pAb (STJ11107624). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SHC1-Y427 antibody (STJ11107624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SKP2-Ser64 antibody (STJ196425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-Smad3-S208/S213 antibody [5A11] (STJA0019136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of various lysates using (STJ11103777) at 1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Smad3-S423/S425 antibody (STJ11103777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (STJ11102566) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Smad3-S423/S425 antibody [S6MR] (STJ11102566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-Smad3 (S425) Mouse mAb diluted at 1:200

Anti-Phospho-SMAD3-S425 antibody (STJ98875)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser204) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-SMAD3-Ser204 antibody (170-219 aa) (STJ90523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Smad3 (S208) Polyclonal Antibody

Anti-Phospho-SMAD3-Ser208 antibody (174-223 aa) (STJ90906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SMAD3-Ser213 antibody (179-228 aa) (STJ90524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-SMAD3-Ser213 antibody (STJ99595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD3-Ser423/425 antibody (STJ196430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse monoclonal antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-SMAD3-Ser425 antibody (376-425 aa) (STJ90745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HeLa cells, using Phospho-Smad3-T179 Rabbit pAb (STJ114841). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SMAD3-T179 antibody (STJ114841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-Smad3-T179 antibody [1A1] (STJA0019135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

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Western blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody

Anti-Phospho-SMAD3-Thr179 antibody (145-194 aa) (STJ90905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from mouse brain tissue, using Phospho-Alpha-Synuclein-S129 Rabbit pAb (STJ22404). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SNCA-S129 antibody (STJ22404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human brain, using Synuclein (Phospho-Ser129) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-SNCA-Ser129 antibody (91-140 aa) (STJ90418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-Synuclein-Alpha (Y125) Polyclonal Antibody

Anti-Phospho-SNCA-Tyr125 antibody (91-140 aa) (STJ91284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from 293 cells treated with Etoposide 25 muM 60', using Synuclein-alpha (Phospho-Tyr133) Antibody. The lane on the left is blocked with the phospho peptide.

Anti-Phospho-SNCA-Tyr133 antibody (91-140 aa) (STJ90680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from mouse brain, using Synuclein-alpha (Phospho-Tyr136) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SNCA-Tyr136 antibody (91-140 aa) (STJ90681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Phospho-Alpha-Synuclein-Y133 polyclonal antibody (STJ11101127) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-SNCA-Y133 antibody (STJ11101127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of various lysates, using Phospho-Alpha-Synuclein-Y133 pAb (STJ11105783) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SNCA-Y133 antibody (STJ11105783)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of lysates from Mouse brain, using Phospho-Alpha-Synuclein-Y136 Rabbit pAb (STJ22403) at 1:1000 dilution. Mouse brain were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SNCA-Y136 antibody (STJ22403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-SOX2-Ser250/Ser251 antibody (STJ194747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-Phospho-SPHK1-Ser-225 antibody (STJA0005597)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$496.13

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Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SPHK1-Ser225 antibody (STJA0009903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Ser349 antibody (STJ196432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SQSTM1-Ser403 antibody (STJ196433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SQSTM1-Thr269/Ser272 antibody (STJ196434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody (STJ11103435) at 1:500 dilution. HeLa cells and C6 cells were treated by MG132 (50 Mu M) at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-SQSTM1/p62-S349 antibody (STJ11103435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-Src antibody (200-300) (STJ502687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-Phospho-Src antibody (200-300) {Biotin} (STJ502689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (200-300) {FITC} (STJ502690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (400-500) (STJ502688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-Phospho-Src antibody (400-500) {Biotin} (STJ502691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-Src antibody (400-500) {FITC} (STJ502692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Western blot analysis of lysates from 293T cells, using Phospho-Src-S17 Rabbit pAb (STJ113487) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SRC-S17 antibody (STJ113487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-SRC-Ser17 antibody (STJ196435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of COLO cells using Phospho-c-Src (S75) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Ser75 antibody (41-90 aa) (STJ90958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SRC-Tyr-215 antibody (STJA0005602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$496.13

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Anti-Phospho-SRC-Tyr-530 antibody (C-Term) [M261] (STJA0005603)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$496.13

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using Src (Phospho-Tyr216) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SRC-Tyr216 antibody (181-230 aa) (STJ90611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of Jurkat cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SRC-Tyr419 antibody (386-435 aa) (STJ90237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Src (Y419) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SRC-Tyr419 antibody (STJ90446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-SRC-Tyr529 antibody (487-536 aa) (STJ90238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (STJ11102608) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SRC-Y419 antibody [S8MR] (STJ11102608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (STJ22407) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SRC-Y529 antibody (STJ22407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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