Immune System | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody

Anti-Phospho-EIF4E-Ser209 antibody (168-217 aa) (STJ90254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Phospho-eIF4E-Ser209 antibody [PT1400R] (STJA0040395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (STJ117894) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-EIF4G1-S1108 antibody (STJ117894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Anti-Phospho-EIF4G1-Ser1108 antibody (STJ196298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eIF4G (Phospho-Ser1108) Antibody

Anti-Phospho-EIF4G1-Ser1148 antibody (1074-1123 aa) (STJ91130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from Jurkat cells, using Phospho-ELK1-S383 Rabbit pAb (STJ22123) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-ELK1-S383 antibody (STJ22123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Ser383) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Ser383 antibody (351-400 aa) (STJ90255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-ELK1-Ser383 antibody (STJA0003626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Canine/Human/Non-Human Primates/Zebrafish

£454.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Ser389) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Ser389 antibody (356-405 aa) (STJ90256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Phospho-ELK1-T417 antibody (STJ11107628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Thr417) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ELK1-Thr417 antibody (379-428 aa) (STJ90257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Phospho-EP300-Ser1834 antibody (STJA0009836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-Phospho-EP300-Ser1834 antibody (STJA0009894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using p300 (Phospho-Ser89) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EP300-Ser89 antibody (55-104 aa) (STJ91120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Phospho-ERK5-T218/Y220 antibody (STJ11107708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Anti-Phospho-EZH2-Thr311 antibody (STJ196302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-FADD-S194 Rabbit pAb (STJ28382). Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-FADD-S194 antibody (STJ11101092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of MOUSE-KIDNEYmouse brainmouse spleen MOUSE-LUNG using p-FADD (S194) antibody. Antibody was diluted at 1:1000

Anti-Phospho-FADD-Ser194 antibody (159-208 aa) (STJ90788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of RAT-HEART cells using Phospho-FANCA (S1149) Polyclonal Antibody diluted at 1：500

Anti-Phospho-FANCA-Ser1149 antibody (1121-1170 aa) (STJ91103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of U2OS cells using Phospho-FANCG-S383 Rabbit pAb (STJ11100974) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-FANCG-S383 antibody (STJ11100974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of K562 cells using Phospho-FANCG (S383) Polyclonal Antibody

Anti-Phospho-FANCG-Ser383 antibody (STJ90615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-Phospho-FBXW7-T205 antibody (STJ11107696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FCGR2B-Tyr292 antibody (258-307 aa) (STJ90485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western Blot analysis of Raji using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-FGR-Tyr412 antibody (STJA0009900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Phospho-Filamin B-2478 antibody (2450-2500) (STJ502437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

Choose Options

Anti-Phospho-Filamin B-2478 antibody (2450-2500) {Biotin} (STJ502435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£584.50

Choose Options

Anti-Phospho-Filamin B-2478 antibody (2450-2500) {FITC} (STJ502436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£584.50

Choose Options

Anti-Phospho-Filamin B-2481 antibody (2450-2500) (STJ502440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

Choose Options

Anti-Phospho-Filamin B-2481 antibody (2450-2500) {Biotin} (STJ502438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£584.50

Choose Options

Anti-Phospho-Filamin B-2481 antibody (2450-2500) {FITC} (STJ502439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£584.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Filamin A-S2152 Rabbit pAb (STJ117881) at 1:1000 dilution. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-FLNA-S2152 antibody (STJ117881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of various cells using Phospho-Filamin 1 (S2152) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-FLNA-Ser2152 antibody (2121-2170 aa) (STJ90760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

Choose Options

Western Blot analysis of mouse brain, rat brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FLT3-Tyr589/591 antibody (STJ196304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Anti-Phospho-FLT3-Tyr591 antibody (STJ196305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using FLT3 (Phospho-Tyr599) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FLT3-Tyr599 antibody (565-614 aa) (STJ90464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

Choose Options

Western blot analysis of 293T-UV cells using Phospho-Flt-3 (Y842) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-FLT3-Tyr842 antibody (808-857 aa) (STJ90580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using FLT3 (Phospho-Tyr969) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FLT3-Tyr969 antibody (935-984 aa) (STJ90944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (STJ110865) at 1:500 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-FLT3-Y589/591 antibody (STJ110865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Anti-Phospho-FLT3-Y842 antibody (STJ11107643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

Choose Options

Western blot analysis of various cells using Phospho-c-Fos (S32) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-FOS-Ser32 antibody (15-64 aa) (STJ90602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various cells using Phospho-c-Fos (S362) Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-FOS-Ser362 antibody (331-380 aa) (STJ90791)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Fos (Phospho-Ser374) Antibody

Anti-Phospho-FOS-Ser374 antibody (331-380 aa) (STJ91255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from HepG2 cells, using Phospho-c-Fos-T232 Rabbit pAb (STJ22143) at 1:1000 dilution or c-Fos antibody (STJ111014471). HepG2 cells were treated by 10% FBS for after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-FOS-T232 antibody (STJ22143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using FOS (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FOS-Thr232 antibody (201-250 aa) (STJ90964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of HUVEC cells treated with EGF 200nM 5', using FosB (Phospho-Ser27) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FOSB-Ser27 antibody (12-61 aa) (STJ91338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-FoxO1-S256 Rabbit pAb (STJ22144). 293T were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-FOXO1-S256 antibody (STJ22144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-FOXO1-S319 Rabbit pAb (STJ11101126) at 1:1000 dilution or FOXO1 antibody (STJ115801). HeLa cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-FOXO1-S319 antibody (STJ11101126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western Blot analysis of HepG2 mouse brain tissue, rat brain tissue, using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-FOXO1-Ser249 antibody (STJA0009891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options