Anti-ITPR3 antibody (1630-1870) (STJ114939)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ITPR3 (1630-1870) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ITPR3 |
| Gene ID | 3710 |
| Uniprot ID | ITPR3_HUMAN |
| Immunogen Region | 1630-1870 |
| Immunogen Sequence | EGSEAYQRCESGGFLSKLIQ HTKDLMESEEKLCIKVLRTL QQMLLKKTKYGDRGNQLRKM LLQNYLQNRKSTSRGDLPDP IGTGLDPDWSAIAATQCRLD KEGATKLVCDLITSTKNEKI FQESIGLAIHLLDGGNTEIQ KSFHNLMMSDKKSERFFKVL HDRMKRAQQETKSTVAVNMN DLGSQPHEDREPVDPTTKGR VASFSIPGSSSRYSLGPSLR RGHEVSERVQSSEMGTSVL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1870 of human ITPR3 (NP_002215.2). |
Additional Info
| Tissue Specificity | Expressed in intestinal crypt and villus epithelial cells. |
| Post Translational Modifications | Phosphorylated by AKT1 on serine and/or threonine residues. |
| Function | Inositol 1,4,5-trisphosphate-gated calcium channel that, upon 1D-myo-inositol 1,4,5-trisphosphate binding, transports calcium from the endoplasmic reticulum lumen to cytoplasm, thus releasing the intracellular calcium and therefore participates in cellular calcium ion homeostasis. 1D-myo-inositol 1,4,5-trisphosphate binds to the ligand-free channel without altering its global conformation, yielding the low-energy resting state, then progresses through resting-to preactivated transitions to the higher energy preactivated state, which increases affinity for calcium, promoting binding of the low basal cytosolic calcium at the juxtamembrane domain (JD) site, favoring the transition through the ensemble of high-energy intermediate states along the trajectory to the fully-open activated state. Upon opening, releases calcium in the cytosol where it can bind to the low-affinity cytoplasmic domain (CD) site and stabilizes the inhibited state to terminate calcium release. |
| Protein Name | Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr3Ip3 Receptor Isoform 3Ip3r-3Insp3r3Type 3 Inositol 1 -4 -5-Trisphosphate ReceptorType 3 Insp3 Receptor |
| Database Links | Reactome: R-HSA-112043Reactome: R-HSA-114508Reactome: R-HSA-139853Reactome: R-HSA-1489509Reactome: R-HSA-2029485Reactome: R-HSA-2871809Reactome: R-HSA-381676Reactome: R-HSA-4086398Reactome: R-HSA-422356Reactome: R-HSA-5218921Reactome: R-HSA-5578775Reactome: R-HSA-5607763Reactome: R-HSA-9664323Reactome: R-HSA-9717207Reactome: R-HSA-983695 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCytoplasmic VesicleSecretory Vesicle MembraneAlso Localizes At Mitochondria-Associated Membranes (Mams) |
| Alternative Antibody Names | Anti-Inositol 1 -4 -5-Trisphosphate-Gated Calcium Channel Itpr3 antibodyAnti-Ip3 Receptor Isoform 3 antibodyAnti-Ip3r-3 antibodyAnti-Insp3r3 antibodyAnti-Type 3 Inositol 1 -4 -5-Trisphosphate Receptor antibodyAnti-Type 3 Insp3 Receptor antibodyAnti-ITPR3 antibody |
Information sourced from Uniprot.org