• Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Flow cytometric analysis of Hela cells using JAK3 monoclonal antibody (blue) and negative control (red).
  • Western blot analysis using JAK3 monoclonal antibody against Jurkat cell lysate (1).

Anti-JAK3 antibody [5H2] (STJ98188)

SKU:
STJ98188

Current Stock:
Host: Mouse
Applications: WB/IF/FC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tyrosine-protein kinase JAK3 is suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5H2
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JAK3
Gene ID: 3718
Uniprot ID: JAK3_HUMAN
Specificity: JAK3 Monoclonal Antibody detects endogenous levels of JAK3 protein.
Immunogen: Purified recombinant fragment of human JAK3 expressed in E. Coli.
Post Translational Modifications Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Protein Name Tyrosine-Protein Kinase Jak3
Janus Kinase 3
Jak-3
Leukocyte Janus Kinase
L-Jak
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-201556
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-8854691
Reactome: R-HSA-8983432
Reactome: R-HSA-8985947
Reactome: R-HSA-9020558
Reactome: R-HSA-9020958
Reactome: R-HSA-912526
Reactome: R-HSA-9679191
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Jak3 antibody
Anti-Janus Kinase 3 antibody
Anti-Jak-3 antibody
Anti-Leukocyte Janus Kinase antibody
Anti-L-Jak antibody
Anti-JAK3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance