• Immunohistochemistry analysis of paraffin-embedded mouse spleen using JAK1 Rabbit monoclonal antibody (STJ11102738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using JAK1 Rabbit monoclonal antibody (STJ11102738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using JAK1 Rabbit monoclonal antibody (STJ11102738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse lung cells, using JAK1 antibody (STJ11102738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-JAK1 antibody (600-700) [S8MR] (STJ11102738)

SKU:
STJ11102738

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-JAK1 (600-700) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JAK1
Gene ID: 3716
Uniprot ID: JAK1_HUMAN
Immunogen Region: 600-700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human JAK1 (P23458).
Immunogen Sequence: GTLMDYKDDEGTSEEKKIKV ILKVLDPSHRDISLAFFEAA SMMRQVSHKHIVYLYGVCVR DVENIMVEEFVEGGPLDLFM HRKSDVLTTPWKFKVAKQLA S
Tissue Specificity Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Post Translational Modifications Autophosphorylated. Phosphorylated by TYK2 on tyrosine residues in response to type-I interferon signaling, leading to its activation. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling. Ubiquitinated by RNF125.leading to its degradation by the proteasome.
Function Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Kinase partner for the type I interferon receptor IFNAR2. In response to interferon-binding to IFNAR1-IFNAR2 heterodimer, phosphorylates and activates its binding partner IFNAR2, creating docking sites for STAT proteins. Directly phosphorylates STAT proteins but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.
Protein Name Tyrosine-Protein Kinase Jak1
Janus Kinase 1
Jak-1
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-110056
Reactome: R-HSA-112411
Reactome: R-HSA-1169408
Reactome: R-HSA-1266695
Reactome: R-HSA-449836
Reactome: R-HSA-5673001
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-8854691
Reactome: R-HSA-8983432
Reactome: R-HSA-8984722
Reactome: R-HSA-8985947
Reactome: R-HSA-9020558
Reactome: R-HSA-9020591
Reactome: R-HSA-9020956
Reactome: R-HSA-9020958
Reactome: R-HSA-909733
Reactome: R-HSA-912526
Reactome: R-HSA-912694
Reactome: R-HSA-9674555
Reactome: R-HSA-9679191
Reactome: R-HSA-9705462
Reactome: R-HSA-9705671
Reactome: R-HSA-9732724
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Wholly Intracellular
Possibly Membrane Associated
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Jak1 antibody
Anti-Janus Kinase 1 antibody
Anti-Jak-1 antibody
Anti-JAK1 antibody
Anti-JAK1A antibody
Anti-JAK1B antibody

Information sourced from Uniprot.org

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