Immune System | Research Area | St John's Laboratory

Western blot analysis of various lysates, using beta 2 Microglobulin rabbit polyclonal antibody (STJ114279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-B2M antibody (21-119) (STJ114279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of beta 2 Microglobulin in paraffin-embedded human colon carcinoma using beta 2 Microglobulin Rabbit polyclonal antibody (STJ22751) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-B2M antibody (21-119) (STJ22751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-B2M antibody (40-120 aa) (STJ192491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using B4GALT1 antibody (STJ111285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-B4GALT1 antibody (45-210) (STJ111285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using B4GALT1 Rabbit polyclonal antibody (STJ11104586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-B4GALT1 antibody (50-215) (STJ11104586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-B4GALT1 antibody (C-Term) (STJ96372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-BAFF/BLyS/CD257 antibody (134-285 aa) [SRM] (STJ11107340)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using BAIAP2 antibody (STJ27290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BAIAP2 antibody (362-521) (STJ27290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BAIAP2 antibody (STJA0007475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using BATF Rabbit polyclonal antibody (STJ116871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BATF antibody (1-125) (STJ116871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-BATF antibody (10-59 aa) (STJ91819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using BAP31 Rabbit monoclonal antibody (STJ11101799) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCAP31 antibody (101-200) [S9MR] (STJ11101799)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g BAP31 antibody (STJ29136). Western blot was performed from the immunoprecipitate using BAP31 antibody (STJ29136) at a dilution of 1:1000.

Anti-BCAP31 antibody (127-246) (STJ29136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using BAP31 Polyclonal Antibody diluted at 1：2000

Anti-BCAP31 antibody (151-200 aa) (STJ91816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (STJ11103448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Bcl 2 antibody [S8MR] (STJ11103448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates, using Bcl-2 antibody (STJ11103434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Bcl-2 antibody (1-100) [S4MR] (STJ11103434)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £574.50

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Western blot analysis of Bcl-2 at dilution of 1:1000

Anti-Bcl-2 antibody [SM0493] (STJA0010493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Human reactive lymph node stained with anti-BCL-6 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of follicular center B-cells.

Anti-BCL-6 antibody (256-389aa) [ZM22] (STJ180288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £566.50

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Anti-BCL-6 antibody (256-389aa) [ZR280] (STJ180432)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£164.50 - £576.50

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Anti-bcl-6 antibody [GI191E/A8] (STJ16100313)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,041.50 - £1,613.50

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Western blot analysis of extracts of Rat spleen, using Bcl10 antibody (STJ11103798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BCL10 antibody (1-118) (STJ11103798)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from normal (control) and Bcl10 knockout (KO) HeLa cells, using Bcl10 antibody (STJ22772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BCL10 antibody (1-233) (STJ22772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Bcl10 Rabbit monoclonal antibody (STJ11102093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BCL10 antibody (1-233) [S3MR] (STJ11102093)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-BCL10 antibody (111-160 aa) (STJ91833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-BCL10 antibody (C-Term) (STJA0005675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Western blot analysis using Bcl-10 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , MCF-7 (3) and Jurkat (4) cell lysate.

Anti-BCL10 antibody [4F8] (STJ97859)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Anti-BCL10 antibody [EBS-T-001] (STJ16101062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-BCL2 antibody (1-100 aa) [6B5] (STJ96943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of THP-1 cells using 3 Mu g Bcl-2 antibody (STJ27524). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (STJ27524) at a dilution of 1:1000.

Anti-BCL2 antibody (1-100) (STJ27524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of Mouse lung using 3 Mu g Bcl-2 antibody (STJ11101769). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (STJ11101769) at a dilution of 1:1000.

Anti-BCL2 antibody (1-211) [S9MR] (STJ11101769)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Rabbit polyclonal antibody (STJ11104432) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCL2 antibody (1-239) (STJ11104432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of L929 cells using Bcl-2 Rabbit polyclonal antibody (STJ113693) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCL2 antibody (1-239) (STJ113693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 cells using Bcl-2 Rabbit polyclonal antibody (STJ113759) at a dilution of 1:150 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCL2 antibody (1-239) (STJ113759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using Bcl-2 Rabbit polyclonal antibody (STJ22775) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCL2 antibody (1-239) (STJ22775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BCL2 antibody at 1:1,000 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-BCL2 antibody (143 aa N-Term) (STJ140179)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Canine/Monkey

£135.50 - £394.50

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Human reactive lymph node stained with anti-BCL-2 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of mantle B-cells and perifollicular T-cells, and no staining of reactive B-cells in the germinal centers.

Anti-BCL2 antibody (41-54 aa) [124] (STJ180017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £447.50

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Western blot analysis of lysates from 1) mouse-liver, 2) mouse-brain, 3) mouse-lung, 4) mcf-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BCL2 antibody (46-95 aa) (STJ91835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Bcl2 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-BCL2 antibody (50-150 aa) [R03-9E1] (STJA0013008)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£264.50

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Anti-BCL2 antibody (STJ196147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of BCL2 in Jurkat and THP-1 cell lysates using BCL2 antibody (1:500 diluted).Predicted band size:29KDa.Observed band size:26KDa.

Anti-BCL2 antibody (STJ99291)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Silkworm

£144.00 - £348.00

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Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) with DAB staining using Bcl-2 monoclonal antibody.

Anti-BCL2 antibody [8E12] (STJ97860)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/FC/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of L929, using Bcl-XL antibody (STJ11104768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BCL2L1 antibody (1-120) (STJ11104768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of C6 cells using Bcl-XL Rabbit polyclonal antibody (STJ22777) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BCL2L1 antibody (1-80) (STJ22777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Bcl-XL antibody (STJ11101776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BCL2L1 antibody (1-80) [S6MR] (STJ11101776)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using Bcl-x Polyclonal Antibody diluted at 1：500

Anti-BCL2L1 antibody (13-62 aa) (STJ91842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot with Anti-BCL2L1 Antibody (STJ500216) on mouse tissues: Kidney, Brain, liver, Tongue, Spinal cord, Cerebellum and Skeletal muscle. at 1:500 dilution in buffer.

Anti-BCL2L1 antibody (150-200) (STJ500216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-BCL2L1 antibody (150-200) {Biotin} (STJ500217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BCL2L1 antibody (150-200) {FITC} (STJ500218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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