• Immunofluorescence analysis of NIH/3T3 cells using APRT Rabbit polyclonal antibody (STJ115881) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using APRT Rabbit polyclonal antibody (STJ115881) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of Rat lung, using APRT Rabbit polyclonal antibody (STJ115881) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-APRT antibody (1-180) (STJ115881)

SKU:
STJ115881

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APRT (1-180) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APRT
Gene ID: 353
Uniprot ID: APT_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).
Immunogen Sequence: MADSELQLVEQRIRSFPDFP TPGVVFRDISPVLKDPASFR AAIGLLARHLKATHGGRIDY IAGLDSRGFLFGPSLAQELG LGCVLIRKRGKLPGPTLWAS YSLEYGKAELEIQKDALEPG QRVVVVDDLLATGGTMNAAC ELLGRLQAEVLECVSLVELT SLKGREKLAPVPFFSLLQYE
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Protein Name Adenine Phosphoribosyltransferase
Aprt
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-74217
Reactome: R-HSA-9734195
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Adenine Phosphoribosyltransferase antibody
Anti-Aprt antibody
Anti-APRT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance