• Western blot analysis of various lysates, using APEH antibody (STJ28166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts from wild type (WT) and APEH knockout (KO) 293T (KO) cells, using APEH antibody (STJ28166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 100 Mu g extracts of SW480 cells using 3 Mu g APEH antibody (STJ28166). Western blot was performed from the immunoprecipitate using APEH antibody (STJ28166) at a dilution of 1:1000.

Anti-APEH antibody (1-260) (STJ28166)

SKU:
STJ28166

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APEH (1-260) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APEH
Gene ID: 327
Uniprot ID: ACPH_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
Immunogen Sequence: MERQVLLSEPEEAAALYRGL SRQPALSAACLGPEVTTQYG GQYRTVHTEWTQRDLERMEN IRFCRQYLVFHDGDSVVFAG PAGNSVETRGELLSRESPSG TMKAVLRKAGGTGPGEEKQF LEVWEKNRKLKSFNLSALEK HGPVYEDDCFGCLSWSHSET HLLYVAEKKRPKAESFFQTK ALDVSASDDEIARLKKPDQA IKGDQFVFYEDWGENMVSKS IPVLCVLDVESGNISVLEG
Tissue Specificity Expressed in erythrocytes (at protein level).
Function This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Also, involved in the degradation of oxidized and glycated proteins.
Protein Name Acylamino-Acid-Releasing Enzyme
Aare
Acyl-Peptide Hydrolase
Aph
Acylaminoacyl-Peptidase
Oxidized Protein Hydrolase
Oph
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-72764
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Acylamino-Acid-Releasing Enzyme antibody
Anti-Aare antibody
Anti-Acyl-Peptide Hydrolase antibody
Anti-Aph antibody
Anti-Acylaminoacyl-Peptidase antibody
Anti-Oxidized Protein Hydrolase antibody
Anti-Oph antibody
Anti-APEH antibody
Anti-D3F15S2 antibody
Anti-D3S48E antibody
Anti-DNF15S2 antibody

Information sourced from Uniprot.org

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