Immune System | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Human IgG2 using Rabbit Anti-Human IgG2 (Fc) mAb (STJ11107129) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 30s.

Anti-Human IgG2-Fc antibody (99-326 aa) [SRM] (STJ11107129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of human plasma, using Human IgG3 antibody (STJ11103091) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Human IgG3 antibody (50-150) [S1MR] (STJ11103091)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Human IgG4 (Fc) using Rabbit anti Human IgG4 (Fc) mAb (STJ11107372) at dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit ( Exposure time: 3s.

Anti-Human IgG4-Fc antibody (99-327 aa) [SRM] (STJ11107372)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using Human IgM Rabbit monoclonal antibody (STJ11103092) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Human IgM antibody (1-453) [S2MR] (STJ11103092)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from Raji cells, using Human IgM Rabbit pAb (STJ11103627) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Human IgM antibody (213-453) (STJ11103627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-Human IgM antibody (218-453 aa) [SRM] (STJ11106989)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various lysates using HUWE1 Rabbit polyclonal antibody (STJ11103418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HUWE1 antibody (1-100) (STJ11103418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-HUWE1 antibody (STJ191946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-HVCN1 antibody (1-100 aa) (STJ11106115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-HVCN1 antibody (2-100 aa) (STJ13101231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-HVCN1 antibody (STJA0008167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Hydroxy-HIF1A-P564 antibody (STJ11106685)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-ICAM-1 antibody {Biotin} (STJ16101145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Human/Baboon/Chimpanzee/Cynomolgus Monkey/Rhesus

£442.50

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HUVEC whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD54 (ABT-CD54) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HUVECPredicted band size: 58kDaObserved band size: 100kDa

Anti-ICAM1 antibody (100-200 aa) [ABT-CD54] (STJ196917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£174.00 - £252.00

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Anti-ICAM1 antibody (100-200 aa) [ABT149] (STJ196708)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of Raji, using ICAM-1/CD54 antibody (STJ11103840) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ICAM1 antibody (250-480) (STJ11103840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using ICAM-1/CD54 antibody (STJ27566) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-ICAM1 antibody (28-480) (STJ27566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using ICAM-1 monoclonal antibody against ICAM1-hIgGFc transfected HEK293 (1) cell lysate.

Anti-ICAM1 antibody (28-480aa aa) [6G12] (STJ98153)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various cells using ICAM-1 Polyclonal Antibody diluted at 1：500

Anti-ICAM1 antibody (479-528 aa) (STJ93634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using ICAM-1 monoclonal antibody against HUVEC cell lysate.

Anti-ICAM1 antibody (N-Term) (STJ98507)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemistry analysis of paraffin embedded human tonsil using ICAM1 at dilution of 1:200

Anti-ICAM1 antibody [SM5148] (STJA0005148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of 3T3 cells using ICAM-2 Polyclonal Antibody

Anti-ICAM2 antibody (151-200 aa) (STJ93635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ICAM2 Rabbit pAb (STJ24115) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit

Anti-ICAM2 antibody (25-223) (STJ24115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of U-937, using ICAM3 antibody (STJ24116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ICAM3 antibody (270-490) (STJ24116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of NIH-3T3 cells using CD50 Polyclonal Antibody

Anti-ICAM3 antibody (484-533 aa) (STJ92132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100

Anti-ICAM4 antibody (141-190 aa) (STJ99004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ICAM4 antibody (STJ29574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ICAM4 antibody (20-140) (STJ29574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ICAM5 antibody (STJ190911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Mouse kidney, using CD275 Rabbit polyclonal antibody (STJ11105325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ICOSLG antibody (19-256) (STJ11105325)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human kidney using ICOSL Antibody (STJ29160) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ICOSLG antibody (19-256) (STJ29160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-ICOSLG antibody (201-250 aa) (STJ99001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ICOSLG antibody [S5158RM] (STJ11105158)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody (STJ115211). Western blot was performed from the immunoprecipitate using IDH1 antibody (STJ115211) at a dilution of 1:1000.

Anti-IDH1 antibody (1-414) (STJ115211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of PC-12 cells using IDH1 Rabbit polyclonal antibody (STJ24121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDH1 antibody (100-414) (STJ24121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Western blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH1 antibody (N-Term) (STJ96781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human glioma stained with anti-IHD1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infiltrating glioma cells whereas normal glial cells are negative

Anti-IDH1 antibody [ZR7] (STJ180518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£202.50 - £814.50

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Anti-IDH1-R132G-specific antibody [GMab-1] (STJA0022479)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132H-specific antibody [HMab-1] (STJA0022481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132L-specific antibody [LMab-1] (STJA0022480)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-IDH1-R132S-specific antibody [SMab-1] (STJA0022482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Immunohistochemistry analysis of paraffin-embedded mouse lung using IFI16 Antibody (STJ115937) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-IFI16 antibody (1-785) (STJ115937)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-IFI16 Antibody (STJ501395) of THP cell at 1:500 dilution in buffer.

Anti-IFI16 antibody (140-190) (STJ501395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Anti-IFI16 antibody (140-190) {Biotin} (STJ501396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-IFI16 antibody (140-190) {FITC} (STJ501397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Western blot analysis of various lysates using IFI16 Rabbit polyclonal antibody (STJ11105507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : MCF7. Exposure time: 0. 5s.

Anti-IFI16 antibody (500-785) (STJ11105507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HeLa and HepG2 cells, using IFI16 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-IFI16 antibody (731-780 aa) (STJ93639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from rat heart, using IFI27 Rabbit polyclonal antibody (STJ116107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IFI27 antibody (1-100) (STJ116107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IFI27 antibody (1-80 aa) (STJ190336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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