• Immunohistochemistry analysis of paraffin-embedded human liver cancer using C-Reactive Protein (C-Reactive Protein (CRP) ) Rabbit monoclonal antibody (STJ11101541) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse serum, using C-Reactive Protein (C-Reactive Protein (CRP) ) antibody (STJ11101541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Human serum, using C-Reactive Protein (C-Reactive Protein (CRP) ) antibody (STJ11101541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CRP antibody (125-224) [S1MR] (STJ11101541)

SKU:
STJ11101541

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CRP (125-224) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRP
Gene ID: 1401
Uniprot ID: CRP_HUMAN
Immunogen Region: 125-224
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 125-224 of human C-Reactive Protein (CRP) (P02741).
Immunogen Sequence: VEFWVDGKPRVRKSLKKGYT VGAEASIILGQEQDSFGGNF EGSQSLVGDIGNVNMWDFVL SPDEINTIYLGGPFSPNVLN WRALKYEVQGEVFTKPQLWP
Tissue Specificity Found in plasma.
Function Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Protein Name C-Reactive Protein Cleaved Into - C-Reactive Protein(1-205
Database Links Reactome: R-HSA-173623
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-Reactive Protein Cleaved Into - C-Reactive Protein(1-205 antibody
Anti-CRP antibody
Anti-PTX1 antibody

Information sourced from Uniprot.org

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