Haemostasis | Research Area | St John's Laboratory

Western blot with Anti-Phospho-AKT1 Antibody (STJ502089) with PAKT peptide.1:250 dilution in buffer. From 10% SDS gel.

Anti-Phospho-AKT1 antibody (430-480) (STJ502089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£528.50

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Anti-Phospho-AKT1 antibody (430-480) {Biotin} (STJ502090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

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Anti-Phospho-AKT1 antibody (430-480) {FITC} (STJ502091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

£584.50

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Anti-Phospho-AKT1 antibody [M114] (STJA0005252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of extracts of MCF7 cells, using Phospho-AKT1-S124 rabbit monoclonal antibody (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of 293T cells, using Phospho-AKT1-S129 antibody (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of MCF7 cells, using Phospho-AKT1-S129 rabbit monoclonal antibody (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [6F8] (STJ97726)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473 polyclonal antibody (STJ116369) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of Jurka cells, using Phospho-AKT1-S473 antibody (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473 monoclonal antibody (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser129 antibody (95-144 aa) (STJ90484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-AKT1-Ser129 antibody (STJ99594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-AKT1-T308 antibody (STJ22013) at 1:500 dilution. Jurkat cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ22013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of RD, using Phospho-AKT1-T450 rabbit polyclonal antibody (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of C6 cells, using Phospho-AKT1-T450 rabbit monoclonal antibody (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnightSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Anti-Phospho-AKT1-Thr-34 antibody (STJA0005253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rabbit/Rat

£365.50

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Akt (Y326) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr326 antibody (292-341 aa) (STJ90503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) (STJ502086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) {Biotin} (STJ502087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£584.50

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Anti-Phospho-alpha Actinin 4-S159 antibody (140-190) {FITC} (STJ502088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£584.50

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Western blot with Anti-Phospho-Annexin A2 Antibody (STJ502104) on rat 1) Midbrain, 2) Cerebellum, 3) Spinal Cord, 4) Cerebral cortex. Antibody at 1:250 dilution in buffer.

Anti-Phospho-Annexin A2 antibody (1-50) (STJ502104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Annexin A2 antibody (1-50) {Biotin} (STJ502105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Annexin A2 antibody (1-50) {FITC} (STJ502106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-ANXA2-Ser26 antibody (STJ90671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using APLP2 (Phospho-Tyr755) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-APLP2-Tyr755 antibody (714-763 aa) (STJ91239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-APP-T668 antibody (STJ11107619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of mouse brain, using Phospho-APP-T668 antibody (STJ22017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-APP-T668 antibody (STJ22017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-APP-Thr668 antibody (STJ196240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Amyloid-Beta (T743) Polyclonal Antibody

Anti-Phospho-APP-Thr743 antibody (711-760 aa) (STJ91015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARRB1-Ser412 antibody (369-418 aa) (STJ90802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using p130 Cas (Phospho-Tyr165) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BCAR1-Tyr165 antibody (131-180 aa) (STJ90857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-BCAR1-Tyr249 antibody (215-264 aa) (STJ90858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-BCAR1-Tyr410 antibody (376-425 aa) (STJ90765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of PC-12 cells, using Phospho-BCAR1-Y165 antibody (STJ117911) at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-BCAR1-Y165 antibody (STJ117911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 polyclonal antibody (STJ11101096) at 1:1000 dilution or BCAR1 antibody (A18270). HeLa cells were treated by Pervanadate (1 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-BCAR1-Y410 antibody (STJ11101096)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 antibody (STJ117887) at 1:5000 dilution. HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-BCAR1-Y410 antibody (STJ117887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-CAV1-Tyr-14 antibody [M283] (STJA0005314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of various cells using Phospho-Caveolin-1 (Y14) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CAV1-Tyr14 antibody (5-54 aa) (STJ90210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Phospho-Caveolin-1-Y14 rabbit polyclonal antibody (STJ116375) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37 °C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-Phospho-CAV1-Y14 antibody (STJ116375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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