• Western blot analysis of various lysates using ESAM Rabbit polyclonal antibody (STJ114102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ESAM antibody (30-248) (STJ114102)

SKU:
STJ114102

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ESAM (30-248) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ESAM
Gene ID: 90952
Uniprot ID: ESAM_HUMAN
Immunogen Region: 30-248
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-248 of human ESAM (NP_620411.2).
Immunogen Sequence: QLQLHLPANRLQAVEGGEVV LPAWYTLHGEVSSSQPWEVP FVMWFFKQKEKEDQVLSYIN GVTTSKPGVSLVYSMPSRNL SLRLEGLQEKDSGPYSCSVN VQDKQGKSRGHSIKTLELNV LVPPAPPSCRLQGVPHVGAN VTLSCQSPRSKPAVQYQWDR QLPSFQTFFAPALDVIRGSL SLTNLSSSMAGVYVCKAHNE VGTAQCNVTLEVSTGPGAA
Tissue Specificity Highly expressed in endothelial cells.
Function Can mediate aggregation most likely through a homophilic molecular interaction.
Protein Name Endothelial Cell-Selective Adhesion Molecule
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Junction
Adherens Junction
Tight Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Endothelial Cell-Selective Adhesion Molecule antibody
Anti-ESAM antibody
Anti-UNQ220 antibody
Anti-PRO246 antibody

Information sourced from Uniprot.org

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