Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and 293T transfected with Phospho-CD115/CSF-1R-Y723, using Phospho-CD115/CSF-1R-Y723 (STJ11101113) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CSF1R-Y723 antibody (STJ11101113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

Choose Options

Western blot analysis of lysates from 293F cells using Phospho-Casein Kinase 2 alpha (CSNK2A1) -T360/S362 Rabbit pAb (STJ22081) at 1:400 dilution. 293F cells were treated by ERK2 (100ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CSNK2A1-T360/S362 antibody (STJ22081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

Choose Options

Western blot analysis of HeLa cells using Phospho-Casein Kinase II Alpha (Y255) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CSNK2A1-Tyr255 antibody (221-270 aa) (STJ90551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of HuvEc cells using Phospho-Casein Kinase II Beta (S209) Polyclonal Antibody

Anti-Phospho-CSNK2B-Ser209 antibody (166-215 aa) (STJ90892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-Beta-Catenin-S33/S37 Rabbit mAb (STJ11102571) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CTNNB1-S33/S37 antibody [S1MR] (STJ11102571)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Beta-Catenin-S33/S37/T41 Rabbit pAb (STJ11100967) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-CTNNB1-S33/S37/T41 antibody (STJ11100967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Beta-Catenin-S45 Rabbit pAb (STJ29346) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-S45 antibody (STJ29346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of various lysates using Phospho-Beta-Catenin-S552 Rabbit pAb (STJ29345) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-CTNNB1-S552 antibody (STJ29345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Beta-Catenin-S552 antibody (STJ11105815) at 1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-CTNNB1-S552 antibody [S5815RM] (STJ11105815)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Beta-Catenin-S675 Rabbit pAb (STJ117893) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-CTNNB1-S675 antibody (STJ117893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Ser33 antibody (17-66 aa) (STJ90206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Catenin-Beta (S552) Polyclonal Antibody.

Anti-Phospho-CTNNB1-Ser552 antibody (STJ91364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CTNNB1-Ser675 antibody (STJ196478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from SW626 cells using Phospho-Beta-Catenin-T41/S45 Rabbit pAb (STJ22085). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-T41/S45 antibody (STJ22085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Western blot analysis of various cells using Phospho-Catenin-Beta (T41/S45) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CTNNB1-Thr41/S45 antibody (11-60 aa) (STJ90208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-CTNNB1-Tyr-142 antibody (STJA0005304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Anti-Phospho-CTNNB1-Tyr-333 antibody (STJA0005305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

Choose Options

Anti-Phospho-CTNNB1-Tyr-489 antibody (STJA0005306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Anti-Phospho-CTNNB1-Tyr-654 antibody (STJA0005307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

Choose Options

Anti-Phospho-CTNNB1-Tyr-86 antibody (STJA0005303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Tyr489 antibody (455-504 aa) (STJ90928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Western blot analysis of 293 cells using Phospho-Catenin-Beta (Y654) Polyclonal Antibody

Anti-Phospho-CTNNB1-Tyr654 antibody (620-669 aa) (STJ90209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from 293 cells, using Phospho-Beta-Catenin-Y333 Rabbit pAb (STJ22086). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CTNNB1-Y333 antibody (STJ22086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£126.50 - £618.50

Choose Options

Anti-Phospho-Cyclin B1-Ser126 antibody [R05-3B3] (STJA0035057)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£140.50 - £308.50

Choose Options

Anti-Phospho-Cyclin E1-Thr77 antibody [R02-6U7] (STJA0036119)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

Choose Options

Western blot analysis of 293 cells using Phospho-Daxx (S668) Polyclonal Antibody

Anti-Phospho-DAXX-Ser668 antibody (634-683 aa) (STJ90785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CHOP (Phospho-Ser30) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DDIT3-Ser30 antibody (15-64 aa) (STJ91075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DDIT4-Thr23/25 antibody (STJA0003765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£488.50

Choose Options

Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DGCR8-Ser377 antibody (STJA0003611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£488.50

Choose Options

Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-S714 Rabbit pAb (STJ22088). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-DNMT1-pS714 antibody (STJ22088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

Choose Options

Anti-Phospho-DYRK2-Tyr382/264 antibody (STJA0009950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-E2F1-Thr433 antibody (388-437 aa) (STJ90458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-S1070 Rabbit pAb (STJ22101) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-EGFR-S1070 antibody (STJ22101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

Choose Options

Anti-Phospho-EGFR-Ser-1142 antibody (STJA0005396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Anti-Phospho-EGFR-Ser-1142 antibody (STJA0041202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

Choose Options

Anti-Phospho-EGFR-Ser-967 antibody (STJA0005397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

Choose Options

Anti-Phospho-EGFR-Ser-967 antibody (STJA0041201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

Choose Options

Western blot analysis of lysates from HeLa cells treated with TSA 400nM 24H, using EGFR (Phospho-Ser1026) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1026 antibody (991-1040 aa) (STJ90567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-EGFR-Ser1036/1037 antibody (STJ196289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Ser1070) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1070 antibody (1041-1090 aa) (STJ90246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from HepG2 cells treated with serum 20% 15', using EGFR (Phospho-Ser1071) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EGFR-Ser1071 antibody (1036-1085 aa) (STJ90568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of 293-EGF cells using Phospho-EGFR (S695) Polyclonal Antibody

Anti-Phospho-EGFR-Ser695 antibody (661-710 aa) (STJ90566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-EGFR-Ser695 antibody [R06-9F3] (STJA0036089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

Choose Options

Western blot analysis of lysates from A-431 and HUVEC cells, using Phospho-EGFR-T669 Rabbit pAb (STJ22097) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-EGFR-T669 antibody (STJ22097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£126.50 - £618.50

Choose Options

Anti-Phospho-EGFR-Thr669 antibody (STJ196290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Phospho-EGFR (T678) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-EGFR-Thr678 antibody (651-700 aa) (STJ90686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Anti-Phospho-EGFR-Thr678 antibody [R05-7H5] (STJA0033911)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

Choose Options