Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of lysates from MCF7 cells, using Phospho-TSC2-T1462 Rabbit pAb (STJ11101006) at 1:1000 dilution. MCF7 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-TSC2-T1462 antibody (STJ11101006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of 293 using p-Tuberin (T1462) antibody.

Anti-Phospho-TSC2-Thr1462 antibody (1428-1477 aa) (STJ91128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of TSC2 (Phospho-Tyr1571) Antibody. The lane on the right is blocked with the TSC2 (Phospho-Tyr1571) peptide.

Anti-Phospho-TSC2-Tyr1571 antibody (1537-1586 aa) (STJ91276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using UBF (Phospho-Ser484) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-UBTF-Ser484 antibody (451-500 aa) (STJ90997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HT29 cells treated with heat shock, using Vitamin D Receptor (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VDR-Ser208 antibody (181-230 aa) (STJ90730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of COLO cells using Phospho-VDR (S51) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-VDR-Ser51 antibody (16-65 aa) (STJ90600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells treated with etoposide 25uM 24h, using Werner Syndrome Helicase (Phospho-Ser1141) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-WRN-Ser1141 antibody (1107-1156 aa) (STJ90561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-WWTR1-Ser89 antibody (STJ194751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-YAP1-S127 Rabbit pAb (STJ29333) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-YAP1-S127 antibody (STJ29333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells using Phospho-YAP1-S128 Rabbit pAb (STJ11103727) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-YAP1-S128 antibody (STJ11103727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of various lysates using phospho-YAP1-S397 Rabbit pAb (STJ11101056) at 1:1000 dilution. NIH3T3 cells and C6 cells were treated by Sorbitol (0. 2M) at 37 °C for 60 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-YAP1-S397 antibody (STJ11101056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Anti-Phospho-YAP1-Ser109 antibody (STJ196474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of Hela cell. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-YAP1-Ser127 antibody (93-142 aa) (STJ90868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-YAP1-Ser397 antibody (STJ196475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 15', using YB1 (Phospho-Ser102) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YBX1-Ser102 antibody (68-117 aa) (STJ90649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-YWHAQ-Ser232 antibody (196-245 aa) (STJ91063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa cells, using Phospho-YWHAZ-S58 Rabbit pAb (STJ22447). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-YWHAZ-S58 antibody (STJ22447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using Phospho-14-3-3 Zeta (S58) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-YWHAZ-Ser58 antibody (24-73 aa) (STJ91013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YWHAZ-Thr232 antibody (196-245 aa) (STJ90869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells using PIDD antibody (STJ24420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIDD antibody (1-85) (STJ24420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PIDD1 antibody (STJA0008026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

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Immunofluorescence analysis of MCF-7 cells using Pin1 antibody (STJ115621). Blue: DAPI for nuclear staining.

Anti-PIN1 antibody (1-163) (STJ115621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using Pin1 Rabbit polyclonal antibody (STJ25001) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIN1 antibody (1-163) (STJ25001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using PIN1 antibody (STJ11104850) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PIN1 antibody (1-163) [S4850RM] (STJ11104850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-PIN1 antibody (1-50 aa) (STJ95095)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from Rat skeletal muscle, using PINK1 Rabbit pAb (STJ29211) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PINK1 antibody (282-581) (STJ29211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PINK1 antibody (Internal Region) (STJA0005787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$628.59

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Western Blot analysis of various cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-PINK1 antibody (STJ192141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-PINK1 antibody (STJ502504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$589.95

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Anti-PINK1 antibody [PT0824R] (STJA0039772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-PINK1 antibody {Biotin} (STJ502505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$729.33

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Anti-PINK1 antibody {FITC} (STJ502506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

$729.33

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Anti-PINP antibody (Met1-Glu92) [2F2] (STJA0018418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human

$309.81 - $440.91

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Immunofluorescence analysis of A549 cells using PIP4K2A antibody (STJ29582).

Anti-PIP4K2A antibody (120-350) (STJ29582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-PIP4K2A antibody (STJA0008345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using PIP4K2B Rabbit pAb (STJ110322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PIP4K2B antibody (1-270) (STJ110322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Anti-PIP4K2B antibody (STJA0007839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PIP4K2C antibody (262-312 aa) (STJ195665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

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Anti-PIP4P1 antibody (STJA0007401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using Pituitary homeobox 2 (PITX2) antibody (STJ25006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PITX2 antibody (1-100) (STJ25006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of HeLa, using Pituitary homeobox 2 (PITX2) antibody (STJ11104743) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PITX2 antibody (1-100) [S4743RM] (STJ11104743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Anti-PITX2 antibody (142-192) (STJA0006624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-PIWIL1 antibody (STJA0007303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-PIWIL1 antibody [SRM13598] (STJ11108328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Mouse/Rat

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Western blot analysis of Mouse testis, using PIWIL2 Rabbit polyclonal antibody (STJ11105678) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PIWIL2 antibody (100-200) (STJ11105678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PIWIL2 antibody (754-804 aa) (STJ193752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PIWIL4 antibody (108-158 aa) (STJ196060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using PIWIL4 antibody (STJ115167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-PIWIL4 antibody (260-460) (STJ115167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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