| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIWIL4 (260-460) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIWIL4 |
| Gene ID: |
143689 |
| Uniprot ID: |
PIWL4_HUMAN |
| Immunogen Region: |
260-460 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIWIL4 (NP_689644.2). |
| Immunogen Sequence: |
SADVSYKVLRNETVLEFMTA LCQRTGLSCFTQTCEKQLIG LIVLTRYNNRTYSIDDIDWS VKPTHTFQKRDGTEITYVDY YKQQYDITVSDLNQPMLVSL LKKKRNDNSEAQLAHLIPEL CFLTGLTDQATSDFQLMKAV AEKTRLSPSGRQQRLARLVD NIQRNTNARFELETWGLHFG SQISLTGRIVPSEKILMQDH I |
| Tissue Specificity | Ubiquitously expressed. Detected in retina, retinal pigment epithelia cells (RPE) (at protein level). |
| Post Translational Modifications | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. |
| Function | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Does not show endonuclease activity. Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus. May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion. Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors. |
| Protein Name | Piwi-Like Protein 4 |
| Database Links | Reactome: R-HSA-5601884 |
| Cellular Localisation | NucleusCytoplasmProbable Component Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During MeiosisPiwil2/Mili Is Required For Nuclear Localization |
| Alternative Antibody Names | Anti-Piwi-Like Protein 4 antibodyAnti-PIWIL4 antibodyAnti-HIWI2 antibodyAnti-PIWI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
