Anti-PIDD antibody (1-85) (STJ24420)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PIDD (1-85) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIDD1 |
| Gene ID | 55367 |
| Uniprot ID | PIDD1_HUMAN |
| Immunogen Region | 1-85 |
| Immunogen Sequence | MAATVEGPELEAAAAAGDAS EDSDAGSRALPFLGGNRLSL DLYPGGCQQLLHLCVQQPLQ LLQVEFLRLSTHEDPQLLEA TLAQL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human PIDD (NP_665893.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Undergoes autoproteolytic processing whose extent either directs cells towards survival or apoptotic pathways. Autoproteolytically cleaved into two main fragments PIDD-N and PIDD-C. PIDD-C can be further processed into PIDD-CC, a processing which is enhanced by DNA damage. The cleavage producing PIDD-C is required for translocation of PIDD1 to the nucleus upon DNA damage and activation of NF-kappa-B. PIDD-CC mediates the interaction with CRADD and the cleavage producing PIDD-CC is required for the activation of CASP2. PIDD-N remains associated with PIDD-C and PIDD-CC after cleavage. |
| Function | Component of the DNA damage/stress response pathway that functions downstream of p53/TP53 and can either promote cell survival or apoptosis. Associated with CRADD and the CASP2 caspase, it forms the PIDDosome a complex that activates CASP2 and triggers apoptosis. Associated with IKBKG and RIPK1, it enhances sumoylation and ubiquitination of IKBKG which is important for activation of the transcription factor NF-kappa-B. |
| Protein Name | P53-Induced Death Domain-Containing Protein 1Leucine-Rich Repeat And Death Domain-Containing Protein Cleaved Into - Pidd-N - Pidd-C - Pidd-Cc |
| Database Links | Reactome: R-HSA-6803207 |
| Cellular Localisation | CytoplasmNucleusEnriched In The Nucleus Upon Dna Damage |
| Alternative Antibody Names | Anti-P53-Induced Death Domain-Containing Protein 1 antibodyAnti-Leucine-Rich Repeat And Death Domain-Containing Protein Cleaved Into - Pidd-N - Pidd-C - Pidd-Cc antibodyAnti-PIDD1 antibodyAnti-LRDD antibodyAnti-PIDD antibody |
Information sourced from Uniprot.org