• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-YAP1-S128 antibody (STJ11103727) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of lysates from HeLa cells using Phospho-YAP1-S128 Rabbit polyclonal antibody (STJ11103727) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-YAP1-S128 antibody (STJ11103727)


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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Short Description: Rabbit polyclonal antibody anti-Phospho-YAP1-S128 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YAP1
Gene ID: 10413
Uniprot ID: YAP1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S128 of human YAP1 (NP_001123617.1).
Immunogen Sequence: HSSPA
Tissue Specificity Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
Post Translational Modifications Phosphorylated by LATS1 and LATS2.leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1.leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes.in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1. Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.
Function Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Protein Name Transcriptional Coactivator Yap1
Yes-Associated Protein 1
Protein Yorkie Homolog
Yes-Associated Protein Yap65 Homolog
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-2028269
Reactome: R-HSA-2032785
Reactome: R-HSA-8939236
Reactome: R-HSA-8940973
Reactome: R-HSA-8951671
Reactome: R-HSA-9619665
Reactome: R-HSA-9796292
Cellular Localisation Cytoplasm
Cell Junction
Both Phosphorylation And Cell Density Can Regulate Its Subcellular Localization
Phosphorylation Sequesters It In The Cytoplasm By Inhibiting Its Translocation Into The Nucleus
At Low Density
Predominantly Nuclear And Is Translocated To The Cytoplasm At High Density
Ptpn14 Induces Translocation From The Nucleus To The Cytoplasm
Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm At The Blastocyst And Epiblast Stages
Alternative Antibody Names Anti-Transcriptional Coactivator Yap1 antibody
Anti-Yes-Associated Protein 1 antibody
Anti-Protein Yorkie Homolog antibody
Anti-Yes-Associated Protein Yap65 Homolog antibody
Anti-YAP1 antibody
Anti-YAP65 antibody

Information sourced from Uniprot.org

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