Anti-PIWIL2 antibody (754-804 aa) (STJ193752)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human PIWL2 at the amino acid range 754-804
General Information
| Short Description | Rabbit polyclonal anti-Piwi-like protein 2 (754-804 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIWIL2 |
| Gene ID | 55124 |
| Uniprot ID | PIWL2_HUMAN |
| Immunogen | Synthesized peptide derived from the human PIWL2 at the amino acid range 754-804 |
| Immunogen Region | 754-804 aa |
| Specificity | This antibody detects endogenous levels of PIWL2 at Human/Mouse |
Additional Info
| Post Translational Modifications | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. |
| Function | Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4. PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded. Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Represses circadian rhythms by promoting the stability and activity of core clock components BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins. |
| Protein Name | Piwi-Like Protein 2Cancer/Testis Antigen 80Ct80 |
| Database Links | Reactome: R-HSA-5601884 |
| Cellular Localisation | CytoplasmPresent In Chromatoid BodyProbable Component Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis |
| Alternative Antibody Names | Anti-Piwi-Like Protein 2 antibodyAnti-Cancer/Testis Antigen 80 antibodyAnti-Ct80 antibodyAnti-PIWIL2 antibodyAnti-HILI antibody |
Information sourced from Uniprot.org