• Immunofluorescence analysis of NIH/3T3 cells, using Pin1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-PIN1 antibody (1-50 aa) (STJ95095)

SKU:
STJ95095

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (1-50 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIN1
Gene ID: 5300
Uniprot ID: PIN1_HUMAN
Immunogen Region: 1-50 aa
Specificity: Pin1 Polyclonal Antibody detects endogenous levels of Pin1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Pin1 at the amino acid range 1-50
Post Translational Modifications Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation. Ser-71 is dephosphorylated upon IL33-stimulation of dendritic cells.
Function Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 1
Peptidyl-Prolyl Cis-Trans Isomerase Pin1
Ppiase Pin1
Rotamase Pin1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-5668599
Reactome: R-HSA-6804756
Reactome: R-HSA-6811555
Reactome: R-HSA-936440
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Colocalizes With Nek6 In The Nucleus
Mainly Localized In The Nucleus But Phosphorylation At Ser-71 By Dapk1 Results In Inhibition Of Its Nuclear Localization
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 1 antibody
Anti-Peptidyl-Prolyl Cis-Trans Isomerase Pin1 antibody
Anti-Ppiase Pin1 antibody
Anti-Rotamase Pin1 antibody
Anti-PIN1 antibody

Information sourced from Uniprot.org

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