Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using INTS6 Rabbit pAb (STJ11106705) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-INTS6 antibody (700-887 aa) (STJ11106705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-INTS7 antibody (364-414 aa) (STJ193718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using INTS7 Rabbit polyclonal antibody (STJ118553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-INTS7 antibody (702-948) (STJ118553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-INTS8 antibody (346-396 aa) (STJ194831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of Mouse brain, using INTS8 antibody (STJ11103355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-INTS8 antibody (600-800) (STJ11103355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using INTS9 Rabbit pAb (STJ112505) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-INTS9 antibody (459-658) (STJ112505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-INTS9 antibody (STJA0007808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of L929 cells using IPO8 Rabbit polyclonal antibody (STJ116881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IPO8 antibody (878-1037) (STJ116881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry of rat liver with Anti-IQSEC3 primary antibody (STJ501485) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-IQSEC3 antibody (1100-1150) (STJ501485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$589.95

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Anti-IQSEC3 antibody (1100-1150) {Biotin} (STJ501486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-IQSEC3 antibody (1100-1150) {FITC} (STJ501487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Western blot analysis of extracts of HeLa cells, using IRAK1 Rabbit monoclonal antibody (STJ11102082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IRAK1 antibody (215-499) [S2MR] (STJ11102082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of various cells using IRAK-1 Polyclonal Antibody

Anti-IRAK1 antibody (353-402 aa) (STJ93756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using IRAK1 antibody (STJ114498) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IRAK1 antibody (424-633) (STJ114498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using ITCH Rabbit mAb (STJ11102343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ITCH antibody (1-100) [S3MR] (STJ11102343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of ITCH in paraffin-embedded mouse brain using ITCH Rabbit polyclonal antibody (STJ11103875) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITCH antibody (1-155) (STJ11103875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITCH antibody (1-155) (STJ118061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$589.95

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Anti-ITCH antibody (250-300) {Biotin} (STJ501507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-ITCH antibody (250-300) {FITC} (STJ501508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Western blot analysis of lysates from mouse brain, using ITCH Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ITCH antibody (386-435 aa) (STJ91517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of CD41/ITGA2B in paraffin-embedded human appendix using CD41/ITGA2B Rabbit monoclonal antibody (STJ11101345) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGA2B antibody (1-100) [S5MR] (STJ11101345)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of HEL cells using CD41/ITGA2B Rabbit polyclonal antibody (STJ11103826) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ITGA2B antibody (160-260) (STJ11103826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-ITGA2B antibody (STJA0010141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, CD41 monoclonal antibody (Q90) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ITGA2B antibody [Q90] (STJ97008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$67.62 - $360.18

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Western blot analysis of K562 cells using Integrin Alpha 4 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ITGA4 antibody (571-620 aa) (STJ96613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of Jurkat, using Integrinalpha4 (ITGA4/CD49d) antibody (STJ24244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITGA4 antibody (770-970) (STJ24244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded mouse testis using Integrin alpha 4 (ITGA4/CD49d) (ITGA4/CD49d) Rabbit monoclonal antibody (STJ11101987) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGA4 antibody (933-1032) [S7MR] (STJ11101987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts from wild type (WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITGA5 antibody (42-995 aa) [S4927RM] (STJ11104927)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104662) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ITGA5 antibody (42-995) (STJ11104662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of Hela cells using Integrin Alpha 5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGA5 antibody (561-610 aa) (STJ97284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Human placenta using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ27965) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ITGA5 antibody (750-1000) (STJ27965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis using Integrin Alpha 5 monoclonal antibody against HeLa, HUVEC cell lysate.

Anti-ITGA5 antibody (N-Term) (STJ98510)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat/Cow

$205.62 - $496.80

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Western blot analysis using Integrin Alpha 5 monoclonal antibody against ITGA5-hIgGFc transfected HEK293 cell lysate.

Anti-ITGA5 antibody [10F6] (STJ98181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Immunohistochemistry analysis of paraffin-embedded human spleen using CD11a/LFA-1A/ITGAL Rabbit monoclonal antibody (STJ11101547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGAL antibody (1071-1170) [S7MR] (STJ11101547)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of 293 cells using Integrin Alpha L Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGAL antibody (171-220 aa) (STJ97273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Polyclonal Antibody (STJ24250) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-ITGAL antibody (26-1086) (STJ24250)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ITGAL antibody (Extracellular region) [M594] (STJA0005445)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$440.91

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Western blot analysis of various lysates using ITGBL1 Rabbit polyclonal antibody (STJ119660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGBL1 antibody (200-300) (STJ119660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ITGBL1 antibody (Met1-Pro494) [1A070] (STJA0021908)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$316.71 - $453.33

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Anti-IWS1 antibody (STJA0007959)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of human-liver tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-JAG1 antibody (981-1030 aa) (STJ96766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using JARID2 Rabbit mAb (STJ11102505) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-JARID2 antibody (100-200) [S5MR] (STJ11102505)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various lysates using JARID2 Rabbit pAb (STJ111865) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-JARID2 antibody (1027-1246) (STJ111865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using JMY Polyclonal Antibody

Anti-JMY antibody (931-980 aa) (STJ93803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using c-Jun Rabbit polyclonal antibody (STJ113779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Jun antibody (1-70) (STJ113779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of human-lung tissue. 1, AP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-JUN antibody (58-107 aa) (STJ91616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using c-Jun Rabbit polyclonal antibody (STJ24273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-JUN antibody (90-230) (STJ24273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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