• Western blot analysis of the lysates from HepG2 cells using Fos antibody.
  • Western blot analysis of 293T cells using c-Fos Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from HeLa cells, using Fos Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, c-Fos Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using c-Fos Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of mouse-kidney tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-kidney tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, c-Fos Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-FOS antibody (331-380 aa) (STJ92252)

SKU:
STJ92252

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein c-Fos (331-380 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOS
Gene ID: 2353
Uniprot ID: FOS_HUMAN
Immunogen Region: 331-380 aa
Specificity: c-Fos Polyclonal Antibody detects endogenous levels of c-Fos protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Fos at the amino acid range 331-380
Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Protein Name Protein C-Fos
Cellular Oncogene Fos
Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit
G0/G1 Switch Regulatory Protein 7
Proto-Oncogene C-Fos
Transcription Factor Ap-1 Subunit C-Fos
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2871796
Reactome: R-HSA-450341
Reactome: R-HSA-6785807
Reactome: R-HSA-6796648
Reactome: R-HSA-9018519
Reactome: R-HSA-9031628
Reactome: R-HSA-9634638
Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Endoplasmic Reticulum
Cytoplasm
Cytosol
In Quiescent Cells
Present In Very Small Amounts In The Cytosol
Following Induction Of Cell Growth
First Localizes To The Endoplasmic Reticulum And Only Later To The Nucleus
Localization At The Endoplasmic Reticulum Requires Dephosphorylation At Tyr-10 And Tyr-30
Alternative Antibody Names Anti-Protein C-Fos antibody
Anti-Cellular Oncogene Fos antibody
Anti-Fos Proto-Oncogene - Ap-1 Transcription Factor Subunit antibody
Anti-G0/G1 Switch Regulatory Protein 7 antibody
Anti-Proto-Oncogene C-Fos antibody
Anti-Transcription Factor Ap-1 Subunit C-Fos antibody
Anti-FOS antibody
Anti-G0S7 antibody

Information sourced from Uniprot.org

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