• Immunofluorescence analysis of U2OS cells using FOXO4 Rabbit polyclonal antibody (STJ23706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using FOXO4 Rabbit polyclonal antibody (STJ23706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using FOXO4 Rabbit polyclonal antibody (STJ23706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using FOXO4 Rabbit polyclonal antibody (STJ23706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using (STJ23706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-FOXO4 antibody (230-341) (STJ23706)

SKU:
STJ23706

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FOXO4 (230-341) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOXO4
Gene ID: 4303
Uniprot ID: FOXO4_HUMAN
Immunogen Region: 230-341
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-341 of human FOXO4 (NP_005929.2).
Immunogen Sequence: SPVGHFAKWSGSPCSRNREE ADMWTTFRPRSSSNASSVST RLSPLRPESEVLAEEIPASV SSYAGGVPPTLNEGLELLDG LNLTSSHSLLSRSGLSGFSL QHPGVTGPLHTY
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Post Translational Modifications Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Protein Name Forkhead Box Protein O4
Fork Head Domain Transcription Factor Afx1
Database Links Reactome: R-HSA-198693
Reactome: R-HSA-5674400
Reactome: R-HSA-5689880
Reactome: R-HSA-9614399
Reactome: R-HSA-9614657
Reactome: R-HSA-9615017
Reactome: R-HSA-9617629
Reactome: R-HSA-9617828
Reactome: R-HSA-9733709
Cellular Localisation Cytoplasm
Nucleus
When Phosphorylated
Translocated From Nucleus To Cytoplasm
Dephosphorylation Triggers Nuclear Translocation
Monoubiquitination Increases Nuclear Localization
When Deubiquitinated
Alternative Antibody Names Anti-Forkhead Box Protein O4 antibody
Anti-Fork Head Domain Transcription Factor Afx1 antibody
Anti-FOXO4 antibody
Anti-AFX antibody
Anti-AFX1 antibody
Anti-MLLT7 antibody

Information sourced from Uniprot.org

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